- 期刊
台灣金線連之天麻素萃取方法
Method for Extraction of Gastrodin in Anoectochilus formosanus Hayata
摘要
天麻素（gastrodin）屬於酚類化合物，也是台灣金線連重要的次級代謝物質之一，本研究之主要目的，在於建立台灣金線連的天麻素萃取技術及比較其在不同器官中的含量變化。試驗結果顯示，鮮品材料先經冷凍乾燥預處理，再用純水作為溶劑並設定溫度在50℃時，為萃取天麻素最適宜的條件。以純水作為溶劑的天麻素平均萃取量（300.8 μg‧g^(-1) ± 43.6 μg‧g^(-1)）高於使用99.9%甲醇溶劑、95%乙醇溶劑和50%乙醇溶液者。台灣金線連植株不同器官中，以根系的天麻素含量最高（2241.7 μg‧g^(-1) ± 331.8 μg‧g^(-1)）且依序大於花朵（660.0 μg‧g^(-1) ± 179.1 μg‧g^(-1)）、葉片（402.5 μg‧g^(-1) ± 50.8 μg‧g^(-1)）、花序軸（285.0 μg‧g^(-1) ± 35.4 μg‧g^(-1)）或莖（273.3 μg‧g^(-1) ± 33.3 μg‧g^(-1)）等部位。本研究成果未來將可提供量產高優質台灣金線連種苗及研發保健產品之重要參考。
並列摘要
Gastrodin is a phenolic compound. It is one of the important secondary metabolites in Anoectochilus formosanus Hayata. The objectives of this study were to establish the method for extraction of gastrodin from A. formosanus and to compare contents of gastrodin in various parts of plants of A. formosanus. Results showed that the proper procedure for extraction of gastrodin from fresh tissues of A. formosanus was by extraction of gastrodin from freeze-dried tissues at 50℃, using deionizer water as solvent. The content of gastrodin extracted with deionizer water was 300.8 μg‧g^(-1) ± 43.6 μg‧g^(-1), and it was significantly higher than those samples extracted by the solvents of 99.9% methanol, 95% ethanol, and 50% ethanol. The content of gastrodin in A. formosanus roots was 2241.7 μg‧g^(-1), which was significantly higher than gastrodin in the samples of flowers (660.0 μg‧g^(-1) ± 179.1 μg‧g^(-1)), leaves (402.5 μg‧g^(-1) ± 50.8 μg‧g^(-1)), rachis (285.0 μg‧g^(-1) ± 35.4 μg‧g^(-1)) or stems (273.3 μg‧g^(-1) ± 33.3 μg‧g^(-1)). This study indicates that roots of A. formosanus are the best tissues for extraction of gastrodin and that the testing procedure described in this study is useful for selection of A. formosanus plantlets with high contents of gastrodin.