- 期刊
秋水仙素處理對藍紫色蝴蝶蘭Phalaenopsis pulcherrima fma. Coerulea原球體多倍體誘導之影響
Effects of Colchicine Treatments on Polyploidy Induction Using Protocorms of Phalaenopsis pulcherrima fma. coerulea
摘要
本研究以藍紫色原生種蝴蝶蘭Phalaenopsis pulcherrima fma. coerulea無菌播種發芽後約6 wk齡之原球體(protocorm)作為培植體，探討秋水仙素(colchicine)濃度及處理時間對多倍體誘導之影響，並以流式細胞儀與根尖染色檢測苗株之多倍性。結果顯示，在存活率方面，原球體在不同濃度與天數的秋水仙素處理下，以處理1 d存活率最高，達85.5-93.3%，隨著秋水仙素處理天數的增加，存活率有降低的趨勢，且二因子間具有顯著交感效應。在多倍體的誘導方面，原球體以0.1 mM秋水仙素浸漬處理1 d即可誘導出多倍體，隨著處理天數的增加，多倍體的誘導率亦隨之提高，處理8 d之多倍體誘導率可達43.9%，此誘導率與2.5 mM秋水仙素處理原球體8 d多倍體誘導率46.0%相較並無顯著差異，但前者可獲得生長良好且數量較多的多倍體植株。比較2倍體與秋水仙素誘導所得之4倍體植株的園藝性狀，結果顯示4倍體植株具有較大的花徑、果莢、梗徑與子房徑，且葉片較寬短、花梗長度較短以及花朵質地較厚實堅挺等特性。有關4倍體之雜交親和性尚需進一步測試。
並列摘要
Effects of colchicine concentration and exposure time on polyploidy induction, using 6-wk-old protocorms of Phalaenopsis pulcherrima fma. coerulea, were conducted in this study. Ploidy levels of seedlings derived from colchicine treatments were analyzed by flow cytometry and chromosome counting of root tips. Results showed that the highest survival rates of 85.5-93.3% were found on protocorms exposed with various concentrations of colchicine for 1 d. It was found that survival rate decreased along with colchicine exposure time. In addition, both factors of colchicine concentration and exposure time had significant interaction on survival rate of protocorms. Both 0.1 mM colchicine for 1 d and 2.5 mM colchicine for 8 d had the highest polyploidy induction rates of 43.9% and 46.0%, respectively. However, a better growth of protocorms as well as plantlet development was observed from the former treatment. Comparison on horticultural traits of diploid and tetraploid plants showed that tetraploid with lager flowers and seed capsules, thicker diameter of stalk and ovary, shorter length of leaf and stalk, and thicker texture of petal were observed. Although tetraploid plants show better horticultural traits than that of diploid plants, their hybridization affinity needs more cross investigations for further information.