- 期刊
不同製程條件苦茶油對胃部保健功效之研究
The Study of Camellia Oils from Different Process Conditions for Gastric Health Protection
摘要
由於現代人飲食型態改變、生活壓力大以及酗酒等不良習慣，胃腸道功能紊亂成為現今常見的通病，如：消化性潰瘍。苦茶油為台灣常見食用油品，於民間療法中被認為具有胃腸保健之功效。本研究室先前的研究指出，苦茶油具有預防酒精性潰瘍之功效，為進一步證實不同來源或製程之苦茶油是否有相同功效，本研究利用體外（in vitro）及體內（in vivo）試驗模式進行探討，分別利用大鼠胃黏膜細胞（rat normal gastric surface mucous cell line; RGM-1）及BALB/c小鼠。結果顯示，RGM-1細胞以不同苦茶油預處理後皆可減緩乙醇誘導之細胞毒性，最後選擇效果最佳之BLCO苦茶油進行動物試驗。動物實驗結果顯示，乙醇（5 mL kg^(-1) B.W.）誘導1 h後會造成胃黏膜損傷。然而，餵食苦茶油（1 mL kg^(-1) B.W.及2 mL kg^(-1) B.W.）3 wk或Lansoprazole （30 mg kg^(-1) B.W.）1 wk後能有效改善乙醇誘導小鼠之胃損傷，提升黏膜黏液之分泌和抗氧化酵素活性，包括：過氧化氫酶（Catalase）、麩胱甘肽-S-轉移酶（Glutathione S-transferase; GST）、麩胱甘肽過氧化酶（Glutathione peroxidase; GPx）及麩胱甘肽還原酶（Glutathione reductase; GRd）；與減緩酒精誘導胃組織發炎因子COX-2及IL-1β之蛋白表現。綜合上述，苦茶油能藉由降低氧化傷害及發炎作用，減緩乙醇所誘導的胃損傷。因此，本研究成果可作為未來開發苦茶油為胃腸相關機能性產品之參考，以提升本土農產品之經濟價值。
並列摘要
The gastrointestinal disorders such as peptic ulcer become a common problem nowadays, which is due to the dietary habit changes, stressed lives and alcoholism. Camellia oil is commonly used in Taiwan and has been shown with healthy effects for gastrointestinal tract in folk medicine. Our previous study has been demonstrated that camellia oil could prevent the gastric mucosal damage caused by ethanol. This study was aimed to further confirm whether the effects of different sources or processes of camellia oils have the same effect. In this study, the rat normal gastric surface mucous cell line (RGM-1) cells and BALB/c mice were used in ethanol-induced gastric mucosal damage model. The results indicated that the ethanol-induced RGM-1 cell mortalities were significantly increased in those pretreated with camellia oils, and the oil with the highest bioactivity was chosen for further animal experiment. Animal experiments showed that ethanol (5 mL kg^(-1) B.W.) induction for an hour can cause gastric mucosal injury. However, pretreatment of mice with camellia oil (1 mL kg^(-1) B.W. and 2 mL kg^(-1) B.W.) for 3 wk or lansoprazole (30 mg kg^(-1) B.W.) for 1 wk effectively improved ethanol-induced gastric injury, enhanced mucosal mucus secretion, antioxidant enzyme activities including catalase, glutathione S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GRd), and alleviated inflammatory cytokines by suppressing the production of COX-2 and IL-1β induced by ethanol in gastric tissue. In conclusion, the camellia oil could alleviate the ethanol-induced gastric injury by reducing oxidative damage and inflammation. The results of this study can be applied to develop the gastrointestinal-related functional products in the future, and thereby enhance the values of indigenous agricultural products.