- 期刊
Determination of Contents of Total Phenol, Flavonoid, Triterpenoid and Peptide and in Vitro Angiotensin-Converting Enzyme Inhibitory Activity of Bitter Melon Extract
苦瓜萃取物總酚、類黃酮、三萜類、胜肽含量及體外抑制血管收縮素轉化酶能力之研究
摘要
Bitter melon (Momordica charantia L.) is an important vegetable in the tropical countries. It is claimed to have beneficial effects because of its content of bioactive components. Hypertension has become a common global health problem leading to many chronic diseases, such as cardiovascular disease, renal disease and diabetes. The inhibition of the angiotensin-converting enzyme (ACE) can help to regulate hypertension. In this study, we evaluated the ACE inhibitory activity of eight different lines of bitter melon extracts with the expectation for screening the line(s) with the potential of anti-hypertension. The results showed that the highest ACE inhibition rate in the extract of BM141 was 93.00% with IC_(50) value 15.18 mg ML^(-1). According to the study, it could be concluded that bitter melon provided inhibition activity against ACE. The bitter melon containing higher total phenols, peptides and triterpenoids had higher ACE inhibition activity and be regarded as indicator components. Further research is needed to validate the antihypertensive ability in bitter melon using experimental animal models of hypertension.
並列摘要
苦瓜（Momoridica charantia L.）是熱帶國家重要的蔬果作物，苦瓜因富含機能性成分而具有許多保健功能，包括降血壓在內。高血壓是全球關切之健康問題，會衍生許多慢性疾病，如心血管疾病、腎臟病和糖尿病。血管收縮素轉化酶（angiotensin-converting enzyme；ACE）的抑制有助於高血壓的調控，本研究以8種苦瓜品系為原料，測定水萃物體外血管收縮素轉化酶抑制活性，期能協助篩選具調節血壓潛力之苦瓜品系。結果顯示，以BM141苦瓜萃取物的ACE抑制率最高，抑制率為93.00%，IC_(50)為15.18 mg mL^(-1)。因此，根據試驗結果，苦瓜確實具有抑制血管收縮素轉化酶之活性。總酚、胜肽、三萜類含量相對高的苦瓜品系，其血管收縮素轉化酶抑制活性較高，可以作為調節血壓相關之指標成分。有關運用高血壓動物模式評估苦瓜降血壓能力，則有待進一步探討。