- 期刊
雄犬腹腔鏡電燒結紮避孕之探討
The Study of Male Dog Contraception with Laparoscopic Electrocoagulation
摘要
本研究探討應用腹腔鏡電燒結紮技術進行雄犬的避孕，包括：（1）腹腔鏡電燒結紮避孕技術之開發，（2）雙側輸精管電燒結紮前、後精液性狀之變化、（3）單側輸精管電繞結紮後生殖器官之形態病理學變化、（4）單側內精動靜脈電燒結紮後生殖器官之形態病理學變化。雙側輸精管電燒結紮後，每週採精一次至第八週，精波均為澄清透明，除第二至第五週採精離心鏡檢，可見到少於十個無活性精蟲外，之後鏡檢皆看不到精蟲。單側輸精管電燒結紮兩個月後，電燒結紮處均變為細長或斷離，電燒結紮側近副睪尾部的管腔內堆積濕厚精液，有二病例（2/8）分別在副睪頭和副睪體見到精蟲肉芽腫，電燒結紮兩個月後睪丸內精細小管造精細胞的層次和形態正常。單側內精動靜脈電燒結紮後第一、二週可見電繞結紮側之蔓狀血管靜脈叢、睪丸和副睪鬱血腫脹，副睪體部和副睪頭部管腔塌陷、管壁變厚且無精蟲存在，第四週可見睪丸精細小管內造精細胞嚴重壞死，第八週除見到睪丸嚴重萎縮壞死外，副睪尾部管腔也呈塌陷且無精蟲存在，顯示不再具有產生精蟲的能力。由本研究結果顯示，以腹腔鏡進行雄犬輸精管、內精動靜脈電燒結紮，因可造成輸精管不可逆的阻塞，睪丸、副睪萎縮無法產生精蟲，可做為一臨床上有效的雄犬避孕方式。
並列摘要
The purpose of this experiment was to apply the technique of laparoscopic electrocoagulation on the contraception of male dog. The experiment included following items: (1) the development of laparoscopic technique for canine contraception, (2) the examination of semen quality before and after electrocoagulation of vas deferens, (3) the morphopathological study of reproductive organs after unilateral vas deferens electrocoagulation, (4) the morphopathological study of reproductive organs after electrocoagulation of internal spermatic artery and vein. After vas deferens were electrocoagulated, no sperm could be found in the semen sample except that a few (less than ten) sperms could be found in centrifugated semen sample within the first five weeks. Two months after electrocoagulation, the electrocoagulative segments of vas deferens were significantly shrunk or even broken. The epididymal duct near the electrocoagulated area and caudal epididymis were dilated and filled with sperms in the lumen. Two cases (2/8) of sperm granulomas were found in caput and corpus of epididymis. However, spermatogenesis of seminiferous tubules in the testis appeared normal. After the electrocoagulation of internal spermatic artery and vein, the venous pampiniform plexus, testis and epididymis were congested and enlarged for the first two weeks. During this period, sperms had disappeared from epididymal duct of both caput and corpus epididymis. These epididymal ducts were collapsed and the wall became thickening. The spermatogenic cell of semineferous tubules in testes became significantly necrotic at fourth week after electrocoagulation. However, the duct of caudal epididymis still contained sperms until the eighth week post-electrocoagulation. It appeared that the ability of sperm production lost completely after eighth week. According to the results of this experiment, laparoscopic electrocoagulation of vas deferens, internal spermatic artery and vein in male dog could either block the passage of reproductive tract or caused the atrophy of testis and epididymis with no sperm productiion. This technique can be applied for canine contraception in clinic.