- 期刊
鉛對雄性ICR小鼠睪丸之毒害
Testicular Toxicity of Lead in Male ICR Mice
摘要
以0、2.5、5、25、50及100 mg/kg body weight (bw)不同劑量醋酸鉛腹腔注射ICR雄性小鼠，探討不同劑量及時間鉛暴露對雄性小鼠之副睪內精蟲濃度、畸形率、活動力及睪丸、副睪組織病理等之毒害。試驗以腹腔注射高劑量0、100 mg/kg bw醋酸鉛連續處理5天後停藥，於處理後第35天解剖。另以0、2.5、5、25及50 mg/kg bw醋酸鉛腹腔注射每週5天，於第21天解剖及0、2.5、5及25 mg/kg bw鉛相同處理至第35天，比較高劑量與低劑量、不同階段重複暴露後對小鼠睪丸之毒性。結果顯示在高劑量鉛連續5天暴露後，小鼠之副睪精蟲濃度及活動力明顯下降，精蟲畸形率則明顯上升。組織切片下，睪丸生精小管明顯壞死及萎縮，副睪管腔精蟲數目明顯減少且有大量壞死精細胞。重複暴露低劑量2.5、5、25及50 mg/kg bw醋酸鉛經21天後，精蟲濃度及活動力亦隨劑量增加而下降，精蟲畸形率則增加，睪丸及副睪之組織病理傷害亦隨藥劑濃度增加而上升。在處理35天後之睪丸傷害又較連續處理21天更加嚴重。綜合本實驗顯示鉛對雄性小鼠具有明顯之精蟲及睪丸毒性。
並列摘要
Male ICR mice were treated intraperitoneally (i .p) with 0 and 100 mg/kg body weight (bw) of lead acetate for 5 day-repeated dosages and sacrificed on day 35 or 0, 2.5, 5, 25, and 50 mg/kg bw 5 days a week and sacrificed on days 21 and 35, to evaluate the sperm concentration, abnormality, motility, testis and epididymis toxicity in different doses and periods. Experimental results indicated that reduced sperm concentration and motility, and increased the sperm abnormality in the cauda epididymis after 100 mg/kg bw of lead for 5-day repeated dosage treatments. The histopathological effects were extensive spermatocytes and spermatids as well as seminiferous tubular atrophy in the testis. Furthermore, much necrotic cell debris was found in the cauda epididymis lumen with only a few sperms left. A dose-dependent decrease occurred in the sperm concentration, and the sperm abnormality increased as well. The histological scores of the testis and cauda epididymis increased when the dose was gradually increased in the 21 day-repeated dose groups. The testicular toxicity in 35 day-repeated group was more severe than that of 21 day-repeated groups, indicating that lead could cause testicular toxicity in male ICR mice.