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酸漿屬種間雜交之研究（Ⅰ）不同成熟度雜交種胚培養
Improvement of Interspecific Hybridization between Physalis angulata and P. ixocarpa ( ) Embryo Culture of Hybrids at Different Stages
摘要
苦蘵與酸漿雜交不親和無法產生成熟的種子，本實驗以苦蘵為母本與粘果酸漿雜交，以番茄種間雜交胚培養的培養基，可成功的獲得子代植株。授粉後23天採集果實內之種子進行胚培養，苦蘵與粘果酸漿紫色種雜交的未成熟胚有7.4％可產生癒合組織，與黃色種雜交者則有3％。另授粉後27天才採集的種間雜交果，苦蘵與紫色種粘果酸漿雜交的未成熟胚僅剩1.4％可產生癒合組織，而與黃色種雜交者則只有1.1％。另先將苦蘵以秋水仙素處理使其染色體倍加後再與紫色種雜交的亦只有1.0％，苦蘵染色體倍加後與黃色種雜交的亦只有0.9％的未成熟胚可產生癒合組織。胚培養形成癒合組織率雖然不高，然而形成之癒合組織可經由再分化為芽體，而成功獲得雜交的酸漿植株。此外亦可知授粉後23天採集的種子其胚成熟度較授粉後27天的種子更適合進行胚培養。
並列摘要
An unilateral incompatibility mechanism prevents Physalis angulata from serving as the male parent in crosses with Physalis ixocarpa. The media used is the same as that for embryo culture in Lycopersicon sp. Twenty-three days after pollination, embryo callus was produced, up to 7.4%, in the immature embryos of the interspecific cross between Physalis angulata and P. ixocarpa (Purple), and 3.0% between Physalis angulata and P. ixocarpa (Toma Verde). Twenty-seven days after pollination, 1.4% of embryo callus was produced in the immature embryos of the interspecific cross between Physalis angulata and P. ixocarpa (Purple), and 1.1% between Physalis angulata and P. ixocarpa (Toma Verde). Colchicines was used for the treatment of Physalis angulata. When plants regenerated from the chromosome were tetraploid (double-chromosome), 1.0% of embryo callus was produced in the immature embryos of the interspecific cross between Physalis angulata (D) and P. ixocarpa (Purple), and 0.9% between Physalis angulata (D) and P. ixocarpa (Toma Verde). Although percentage of the immature embryos plated for callus was low, this method for production of F1 interspecific hybrids between Physalis angulata and P. ixocarpa is efficient. This paper shows that seeds from the 23-days-old fruits were found with the highest percentage of callus formation.