- 期刊
ADP與Cyclic AMP系統對鱟血膠凝形成之影響
The Role of ADP-Cyclic Amp System in Horseshoe Crab Amebocytes Aggregation and Gel Formation
摘要
以ADP及cyclic AMP相關藥物對內毒素引起鱟膠凝及鱟血球凝集的影響作研究，發現ADP，cyclic AMP，ATP和aminophylline均可抑制由內毒素引起鱟膠凝的反應。相反地，phosphodiesterase卻可顯著地提高鱟膠凝反應速度。另外，calmodulin的拮抗劑trifluoperazine，亦會抑制鱟膠凝之形成，但其對鱟血球凝集反而有誘發作用。綜合本實驗結果，推測cyclic AMP-phosphodiesterase系統在內毒素引起鱟膠凝反應中，扮演一重要角色；此外，鱟試劑中可能有calmodulin-like protein 存在，在內毒素及Ca＋＋同時存在下，藉著激活cyclic AMP-phosphodiesterase途徑而活化膠凝形成。
關鍵字
無資料並列摘要
A study of the effects of ADP and cyclic AMP related drugs on gel formation induced by endotoxin and amebocytes aggregation was conducted. The gel formation induced by endotoxin could be inhibited by ADP, cyclic AMP, ATP and aminophylline. Conversely, Phosphodiesterase enhanced gelation rate. A calmodulin antagonist, trifluoperazine, also inhibited endotoxin-induced gel formation, but this agent was able to induce potentiation in amebocytes aggregation. These results suggest that the cyclic AMP phosphodiesterase system may play an important role in endotoxin-induced gel formation, and it was concluded that, in the presence of both Ca＋＋ and endotoxin, the calmodulin-like protein will activate the cyclic AMP phosphodiesterase system and decrease the concentration of cyclic AMP, thus promoting gel formation.