- 期刊
Detection of EBV in Tumor Tissue and Peripheral Blood by Polymerase Chain Reaction in Patients with Nasopharyngeal Carcinoma
以聚合酶連鎖反應檢測鼻咽癌病人腫瘤組織與末稍血液EB病毒之DNA
摘要
69位尚未接受治療的鼻咽癌病人及18位健康正常者被選擇當做疾病組及控制組，然後利用聚合酶連鎖反應方法檢查此兩組人之鼻咽腔組織及末稍血液之單核球，是否含有EB 病毒之DNA。一條明顯的239bp 帶顯示EBV 之存在。結果顯示有91 . 3 ％之鼻咽癌病人其鼻咽組織含EBV 之DNA ，而控制組則僅含16. 7 %，統計學上顯示有意義的差異。然而，此兩組人的末稍血液單核球，卻都無法檢測出EBV之DNA。此外卻也發現EBV DNA 之存在與否和血清標幟，病理型態，鼻咽腫瘤與頸部淋巴腫瘤大小，以及是否轉移並無關係。雖然我們可以從鼻咽癌病人之鼻咽腔組織發現極高的EB病毒DNA之存在率，但環境及基因的共同因素，仍是必須考慮的。
關鍵字
無資料並列摘要
Sixty-nine untreated patients with a pathologically verified nasopharyngeal carcinoma were selected for the study of detection of EBV in nasopharyngeal tumor and peripheral blood by polymerase chain reaction (PCR). Primers were directed to conserved regions of EBV genorne encoding capsid protein gp 220 (Barn HI L region). A distinct 239 bp band of the PCR products indicated the presence of EBV. Results showed that EBV DNA was obtained in 91.3% of 69 NPC patients and 16.7% of 18 healthy individuals on nasopharyngeal tissue, and the difference was statistically significant between the above two groups. Nevertheless, no EBV DNA was verified from the mononuclear cells of the peripheral blood of the two groups. There was no relationship between the positive EBV DNA and the titer of serological markers. Meanwhile, the positive EBV DNA did not show any relationship with the histology type, tumor and nodal bulk, or even metastasis. Although a high positive rate of EBV DNA was detected in nasopharyngeal tumor of patients, additional environmental and genetic factors must still be considered.