- 期刊
Interleukin-8 Secretion by Cultured Oral Epidermoid Carcinoma Cells Induced with Nicotine and/or Arecoline Treatments
尼古丁和檳榔鹼單獨或共同處理會促使培養之口腔上皮癌細胞分泌白血球間質－八
摘要
白血球間質－八（Interleukin-8, IL-8）是一個多功能的細胞激素。它可以在免疫及發炎活動上扮演色；其他如體外觀察顯示IL-8對角質細胞中為生長因子。本研究以１至l00μ M的nicotine與arecoline，單獨或是共同處理培養的口腔癌 KB CCLl7細胞７２小時，再以酵素連結免疫各應分析（enzyme-linked immunosorbent assay ELISA）來測定細胞上清液中 IL-8的濃度。重覆測定分析變異數來檢測各個實驗組間的差異性。在nicotine和arecoline的共同處理的實驗中，IL-8的濃度被發現會有比對照組戲劇性增加很多。濃度１μM的nicotine和１μM的arecoline共同處理會使IL-8濃度比對照組增加66.7%；l00μ M的nicotine和l00μ M的arecoline共同處理會使 IL-8濃度比對照組增加2.5倍。當外加1μg/ ml的單一純化的 IL-l α或 IL-l β抗體在各個處理中， IL-8濃度會有顯著性減少。在細胞增殖方面， IL-l α或 IL-l β抗體對於對照組並沒有顯著性影響，但是若把 IL-l α或 IL-l β抗體加入nicotine和arecoline的處理中，則其細胞增殖會比未加入抗體者顯著地增加很多。這些結果建議在活體內IL-8的分泌會受到IL-l 局部產生的特殊調節。而且nicotine和arecoline會促使IL-l 分泌去抑制 KB CCLl7細胞的生長。因此，在吸煙和和嚼檳榔所導致之一連串相關細胞激素分泌反應的發炎或致癌作用中，IL-8可能扮演一個重要角色。
關鍵字
無資料並列摘要
Interleukin-8 (IL-8) is one of the multifunctional cytokines that can playa a role on immune and inflammatory activities. Other in vitro observations indicated that IL-8 is a growth factor for keratinocytes. However, as the role of IL-8 in oral cancer cells is unclear, this study is thus designed to examine IL-8 secretion in cultured oral epidermoid carcinoma KB CCL17 cells treated with nicotine and/or arecoline. The cultures were treated with nicotine (1 or l00μ M) and arecoline (l or 100μM), alone or both, for 72 hrs. Enzyme-linked immunosorbent assay (ELISA) was used to examine IL-8 concentrations in culture supernatants. A repeated measure analysis of variance was used to identify differences among the treatments. Nicotine and arecoline, single or combined treatment, increased IL-8 secretion in KB CCLl7 cells. When monoclonal 1μg/ ml of antibody was added against IL-l α or IL-l β in the treatment, IL-8 concentration significantly decreased compared with the non-added one. Exposure of cells to antibody against IL-1α or IL-1β showed no significant increase in cell growth as compared with the control (medium alone). However, incubation of cells for 72 hrs in the presence of nicotine and/or arecoline treatments and antibody against IL-1αor IL-1βsignificantly increased cell growth as compared with the antibody free one. It was concluded that IL-8 secretion by KB CCLl7 cells may be partially mediated by IL-l which could inhibit the KB CCLl7 ell growth. Thus, IL-8 may be a vital participant in the cascade of interacting cytokines during smoking and areca quid chewing, inducing inflammation in oral cancer.