- 期刊
Immunization of Rodents against Hymenolepis Infections Using Non-Viable Homologous Oncospheres
應用不具活性之同種六鉤名幼蟲接種鼠類對抗包膜絛蟲之感染
摘要
接種同種或異種六鉤幼蟲(oncospheres)，可使豬體産生预防台灣絛蟲之免疫反應。本研究之目的在確定接種不具活性之同種包膜條蟲(Hymenolepis)六鉤幼蟲可引起鼠類對缩小包膜絛蟲(H. diminuta)、短小包膜絛蟲(H. nana)及微小包膜絛蟲(H. microstoma)之免疫力。六鉤幼蟲自蟲卵孵出，並储存在-70γC超過一個月。實驗组包括四組Sprague-Dawley大白鼠或ICR小白鼠，每組各以皮下注射接種含有500個不具活性之鉤幼蟲及Freund佐劑混合液一至四次（每次相隔一週），控制組不接種六鉤幼蟲。接種後，每隻動物自口腔感染三隻新鮮具有活性之似囊尾幼蟲，並作解剖檢查。接種後之大白鼠或小白鼠不感染縮小包膜條蟲或短小包膜條蟲，惟34隻小白鼠有28隻感染微小包膜絛蟲。結果顯示，鼠類接種縮小包膜絛蟲或短小包膜絛蟲可對同種包膜絛蟲産生完全免疫力，對於已産生免疫力之宿主並不需要重覆接種。
並列摘要
Immunity to Taiwan Taenia infection in pigs can be stimulated using homologous or heterologous nonviable Taenia oncospheres. This study was designed to determine whether homologous non-viable oncospheres could stimulate immunity to Hymenolepis infection in rodents. Hatched oncospheres were prepared from eggs of Hymenolepis diminuta, Hymenolepis nana, and Hymenolepis microstoma and kept at -70γC for more than 1 month. A mixture of 500 non-viable oncospheres of each tapeworm and complete Freund's adjuvant was injected subcutaneously in four groups of Sprague-Dawley rats or ICR mice one to four times at an interval of 1 week; controls were not immunized. After immunization, each rodent was orally inoculated with three fresh active cysticercoids of H. diminuta or H. microstoma or 500 fresh eggs of H. nana. The animals were then necropsied for adult tapeworms. No rats or mice immunized with non-viable oncospheres of H. diminuta or H. nana were infected by the challenge inoculation. However, 28 of 34 mice immunized with non-viable H. microstoma oncospheres were infected after inoculation with cysticercoids. This study demonstrated complete protection against infection by homologous parasites in rats or mice immunized with non-viable oncospheres of H. diminuta and H. nana, respectively. Repeated immunization may not be required if resistance is stimulated in rodent hosts.