- 期刊
Association between Cigarette Smoking and Hypoxanthine Guanine Phosphoribosyltransferase Activity
抽菸與HGPRT酵素活性的關係
摘要
本研究之目的主要是在探討抽菸行為與HGPRT酵素活性相關之因素。以橫斷式研究法收集82位男性參與者，其中38位非抽菸者，抽菸者有44位。以高效能液態層析法來測量周邊單核細胞所分泌的IMP濃度作為HGPRT酵素活性的指標。所探討的相關因素包括年齡、族別、肝功能指數(GOT、GPT)、膽固醇、尿酸、三酸甘油脂、肌酸酐、身體質量指數、痛風、收縮壓、舒張壓、以及抽菸飲酒行為。結果顯示：所有參與者HGPRT酵素活性的平均值為7.05(±3.44 nmol/10^6、viable cells/h)，抽菸者的HGPRT酵素活性平均位顯著低於非抽菸者(6.24±3.40 vs 7.98±3.28 nmol/10^6 viable cells/h; p=0.02)。此外，HGPRT酵素活性隨著抽菸數目的增加而降低，同時，在調整抽菸的年數及數目後，此酵素活性隨著開始抽菸年齡的增加而增加。結論：抽菸量與HGPRT酵素活性呈負相關，且開始抽菸年齡越大其HGPRT酵素活性越高。
並列摘要
The aim of this study was to investigate the association between smoking behavior and hypoxanthine guanine phosphoribosyltransferase (HGPRT) activity. A cross-sectional study was performed of 82 men, including 38 non-smokers and 44 smokers. Inosine monophosphate (IMP), the product of HGPRT (used as the index of activity), was measured in peripheral blood mononuclear cells using high-performance liquid chromatography. The factors potentially associated with HGPRT activity included age, glutamyl oxaloacetic transaminase, glutamyl pyruvic transaminase, cholesterol, uric acid, triglycerides, creatinine, body mass index, gout, systolic blood pressure, diastolic blood pressure, alcohol consumption, and cigarette smoking. Mean HGPRT activity was 7.05±3.44 nmol/10^6 viable cells/hour for all participants, and was significantly lower for smokers than for non-smokers (6.24±3.40 vs 7.98±3.28 nmol/10^6 viable cells/hour; p=0.02). In addition, as the number of smoked cigarettes increased, the HGPRT activity decreased (p＜0.05). The age at onset of cigarette smoking showed a positive correlation with HGPRT activity after adjusting for smoking duration, serum uric acid, and cigarettes smoked per year using a multiple regression model (p＜0.001). We concluded that the greater the number of cigarettes smoked, the lower the HGPRT activity, and that HGPRT activity was higher in smokers who had started smoking later.