- 期刊
Effects of Zinc Compound on Body Weight and Recovery of Bone Marrow in Mice Treated with Total Body Irradiation
鋅化合物對於接受全身放射線照射小鼠體重與骨髓恢復之影響
摘要
本研究是探討鋅化合物對於放射線所引起體重降低與骨髓抑制之作用是否有影響。研究中之ICR小鼠隨機分為兩組，實驗組餵食鋅化合物而對照組餵食逆滲透純水。餵食藥物七天後小鼠再分組接受高能放射線全身照射，照射劑量為3 Gy與6 Gy並定期紀錄小鼠體重變化。全身照射後第三週與第五週，解剖取出後大腿骨髓進行組織細胞檢測。實驗結果發現，於全身照射後第一週至第五週間，無論餵食藥物與否，6 Gy組平均體重均較3 Gy組輕且存活率較差。（照6 Gy/照3 Gy）平均存活天數為餵食鋅組26天176天而餵食水組14天170天且P值均小於0.00001。而餵食鋅化合物與否對於接受相同劑量全身照射的小鼠而言，餵食鋅組平均體重於照射後第二與三週比餵食水組重7到8公克且P值小於0.05。但有無餵食鋅化合物對於接受相同劑量全身照射的小鼠存活率，則未達統計上意義。從組識病理學檢查可見，接受6 Gy比接受3 Gy全身照射的小鼠骨髓細胞照射後修復較差。餵食水的對照組，接受3 Gy的骨髓中有核細胞及巨核綱胞於第五週可觀察到已恢復，而6 Gy組則尚未恢復。而本研究中餵食鋅化合物的實驗組骨髓細胞均於照射後第三週觀察到已恢復。本研究結論，餵食鋅化合物對於全身照射後小鼠的體重有幫助。而骨髓之組識學檢查亦顯示，餵食鋅化合物的小鼠骨髓細胞照射後修復情況較好。因此我們建議在臨床接受放射治療之病患可以使用鋅化合物，利用鋅化合物之促進細胞組識之恢復作用以期降低放射線副作用。
並列摘要
This study aimed to investigate if zinc compound would have effects on body weight loss and bone marrow suppression induced by total body irradiation (TBI). ICR mice were divided randomly into two groups and treated with test or control compounds. The test compound contained zinc (amino acid chelated with bovine prostate extract), and the control was reverse osmosis pure water (RO water). One week after receiving the treatment, mice were unirradiated, or irradiated with 6 or 3Gy by 6MV photon beams to the total body. Body weight changes were examined at regular intervals. Three and 5 weeks after the radiation, animals were sacrificed to examine the histologic changes in the bone marrow. Lower body weight in the period of 1-5 weeks after radiation and poor survival rate were found after the 6Gy TBI, as compared with the 3Gy groups. The median survival time after 6Gy and 3Gy TBI for mice given the test compound were 26 and 76 days, respectively, and the corresponding figures were 14 and 70 days, respectively, for mice given the control compound (p＜0.00001). With zinc supplement, the mean body weight in mice which received the same dose of radiation was 7-8 g heavier than in the water-supplement groups during the second and third weeks (p＜0.05). Hence, there was no statistically significant difference in survival rate between zinc and water supplement in mice given the same dose of irradiation. Histopathologically, there was less recovery of bone marrow cells in the 6Gy groups compared with the 3Gy groups. In the 3Gy water-supplement group, the nucleated cells and megakaryocytes were recovered in the fifth week when recovery was still not seen in the 6 Gy group. With zinc supplement, these cells were recovered in the third week. In this study, we found that zinc is beneficial to body weight in mice treated with TBI. Histologic examination of bone marrow showed better recovery of bone marrow cells in groups of mice fed with zinc. This study suggests that zinc can be used as supplements in cancer patients receiving radiotherapy to reduce radiation-induced complications.