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應用中性電解次氯酸水噴霧去除溫室作業環境空氣中生物性污染物

APPLICATION OF NEUTRAL ELECTROLYTIC HYPOCHLOROUS ACID WATER SPRAY TO REMOVE AIR BIOLOGICAL POLLUTANTS IN GREENHOUSE OPERATING ENVIRONMENT

摘要


我國的溫室技術已逐漸廣泛應用於如金線蓮等高經濟價值之作物上,然隨其應用程度與範圍提升,潛在危害也應予重視。溫室作為充斥各種生物氣膠發生源之封閉環境,其環境品質令人憂慮。該類空間已被相關研究證實,可能對長期暴露其中的作業人員造成健康危害,亦可能損及內部作物。故對溫室內的抑菌措施即屬值得重視與發展的方向。本研究參酌過往相關研究,以相對降低對人體與作物潛在危害可能之中性電解次氯酸水噴霧技術為核心,檢驗其於實務溫室上的應用成效。過往該中性電解水,已於實驗室內與禽類飼養場等環境中證實其抑菌效果,本研究於此將其應用於植物作物溫室中,檢視其於現地中之效果。本研究使用電解水噴霧系統生成中性次氯酸水,定比稀釋後以超音波震盪成超細粒徑之微粒後噴出自由有效餘氯200 mg/L與100 mg/L的中性次氯酸水噴霧,進行溫室內抑菌程序。後續針對溫室內兩點採樣點分別於上午10點與下午4點進行細菌與真菌生物氣膠採樣檢測。研究成果發現,在中性電解水噴霧系統作用後,並不會立即去除環境內的生物氣膠,然隨著作用時間拉長、次氯酸分子與生物氣膠充分反映後,即會呈現出顯著之抑菌效用。於約90分鐘內的有效作用期間,對細菌與真菌生物氣膠的最大抑菌效用分別為60~83%與56~83%。證明該中性電解次氯酸水噴霧於植物溫室中之抑菌成效。其中,自由有效餘氯100 mg/L的電解水噴霧,其最大抑菌效用確實低於200 mg/L者,特別是在針對真菌的作用上。

並列摘要


The greenhouse is widely used to plan some valuable cash crops in Taiwan. Regarding the greenhouse technology have been widely used, we should pay more attention to its potential risk. As a confined space filled with a variety of bioaerosols sources, the environmental quality of the greenhouse should be worried about. Space like a greenhouse has been already proven that may be damage to human health or plant planting inside by some related studies. According to previous studies, this study chose to apply neutral electrolytic hypochlorous acid water as to activate bioaerosols. This technology has been proven useful in laboratories and poultry houses, and this study would estimate its performance in the greenhouse. This study used the system to generate neutral electrolytic hypochlorous acid water and sprayed them after diluting and vibrating it into aerosols. There were two different concentrations of neutral electrolytic hypochlorous acid water: 200 mg/L and 100 mg/L. The different concentrations made us can compare their performance of inactivating bioaerosols. After spraying neutral electrolytic hypochlorous acid water aerosol, we collected and analyzed the concentration of bacteria and fungi bioaerosol to estimate its performance. The results show that it won't inactivate bioaerosols immediately. However, it showed a significant antibacterial effect as times go by. In its working period which is about 90 minutes, we found up to about 60 ~ 83 % and 56 ~ 83 % of the antibacterial performance for bacteria and fungi. That indicated neutral electrolytic hypochlorous acid water can be used to inactivate bioaerosols in the greenhouse. Besides that, we did find that the inactivating performance of 100 mg/L is weaker than 200 mg/L, for fungi especially.

參考文獻


Chuang, C.Y., Yang, S., Chang, M.Y., Huang, H.C., Luo, C.H., Hung, P.C., and Fang, W. “Inactivation efficiency to Bacillus subtilis and Escherichia coli bacterial aerosols of spraying neutral electrolyzed water.” Journal of the Air and Waste Management Association, 63(12), 1447-1456, 2013.
Chuang, C.Y., Yang, S., Huang, H.C., Luo, C.H., Fang, W., Hung, P.C., and Chung, P.R., “Applying the Membrane-less Electrolyzed Water Spraying for Inactivating bioaerosols.” Aerosol and Air Quality Research, 13, 350-359, 2003.
Huang, Y.R., Hung, Y.C., Hsu, S.Y., Huang, Y.W., and Hwang, D.F., “Application of Electrolyzed Water in the Food Microbiology.” Food Control, 19, 329-45, 2008.
Kurahashi, M., Ito, T., Naka, A. “Spatial Disinfection Potential of Slightly Acidic Electrolyzed Water”. Plos one, 16(7), e0253595, 2021.
Naka, A., Yakubo, M., Nakamura, K., & Kurahashi, M.“Effectiveness of Slightly Acidic Electrolyzed Water on Bacteria Reduction: in vitro and Spray Evaluation”. PeerJ, 8, e8593. 2020.

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