The environmental problems demanded to use bio-material in several industrial applications. In this case, the use of biological material in composite structure have been increased in this century. The main idea of this research is to use a bio-based material in sandwich composite structure dedicated to transport industrial applications. Also, auxetic structures have presented a good mechanical property such as higher stiffness, strength, and energy absorption. This study presents an experimental analysis of the static and dynamic properties of a bio-sandwich composite with conventional honeycomb and auxetic core. An additive manufacturing technique is used to produce the sandwich composites. Static tensile, static and fatigue three-point bending and vibration tests were used to characterize the tested sandwiches. The effect of the bio-based material, the number of cells in the core as well as the core topology are investigated. The Young modulus and ultimate strength and strain under tensile tests are determined for the two tested material which are the polylactic acid and the polylactic acid reinforced with flax fibers. Also, the static bending stiffness is calculated for each sandwich configurations. The loss factor is calculated under vibration tests. The determined properties in this study, give a large idea on the comportment of bio-sandwich with conventional honeycomb and auxetic core under a diverse test. It was found that sandwich made with polylactic acid and high number of cells presents the highest stiffness. However, sandwich made with polylactic acid reinforced with flax fibers and auxetic core improves the dissipated energy and damping properties.