- 期刊
Evaluation of the Role of Noradrenaline Precursor on LH Release Induced by Ovarian Steroids
正腎上腺素前質對卵巢激素引發黃體生成激素分泌的評估
摘要
本報告為分析血漿中黃體生成激素含量的變化，來推論正腎上腺素性能機制管制性釋素分泌的研究。 將Sprague-Dawley種系的成年雌鼠，在間情期第一日上午十時切除卵巢，隨即處理雌性素，次日上午九時處理正腎上腺素的前質（雙羥絲胺酸），九時半處理助孕素。分析當日下午血中黃體生成激素含量的變動情形，發現會有87.5％的雌鼠呈現黃體生成激素的潮放；但是若在處理雙羥絲胺酸之前30分鐘，先處理血清加壓素的前質（五－羥色胺酸），則會完全抑制雙羥絲胺酸的促進效果。如果將切除卵巢的手術及處理卵巢激素與雙羥絲胺酸的全部過程，順次提早五小時，則在處理絲胺酸後八小時內，血中黃體生成激素濃度未見有改變的跡象，結果說明：雙羥絲胺酸本身或正腎上腺性能機制，只是降低支配性釋素分泌的神經元的閾值。另一方面光照時段是決定性釋素－黃體生成激素大量分泌的重要因素。
關鍵字
無資料並列摘要
Pi,W.P. and C.H.Lin. Evaluation of the role of noradrenaline precursor on LH release induced by ovarian steroids. Chinese J.Physiol.34(3):333-339,1991.The role of noradrenaline precursor on the release of luteinizing hormone-releasing hormone based on the plasma concentration of luteinizing hormone(LH)was studied in rats. Rats were ovariectomized at 1000h on diestrus day 1(D1)and primed with estradiol immediately after the operation. They received progesterone at 0930h in the next morning(the expected diestrus day 2,D2).Dynamic changes of plasma LH levels were examined in the afternoon of D2.Treatments and results are as follows:1)Administration of DL-threo-dihydroxyphenylserine(DOPS,200mg/kg BW,ip)at 0900h on D2,30 min prior to progesterone treatment, results in 87.5% of rats showing LH surges.2)Administration of 5-hydroxytryptophan(5-HTP,100mg/kg BW,ip)30 min before DOPS completely inhibits the action of DOPS on LH release induced by ovarian steroids.3)Making a change of the whole working schedule of operation, estrogen was given 5 hours earlier on D1.DOPS and progesterone were given at 0500 hand 0530h on D2, respectively.It does not induce the LH surge during the whole morning of D2. These results demonstrate that DOPS might just reduce the threshold of the central neurons governing LH release. The time in a fixed lighting schedule was important in determining the LH release induced by ovarian steroids.