Discordance between EGFR Expression and Clinicopathologic Parameters of Colorectal Adenocarcinoma in Taiwan

Wen-Chiuan Tsai ； Chih-Kung Lin ； Herng-Sheng Lee ； Ann Chen ； Shin Nieh ； Cheng-Ping Yu ； Chang-Chieh Wu ； Shu-Wen Jao ； Jong-Shiaw Jin

《The Chinese Journal of Physiology》 55卷5期 (2012/10) Pp. 352-360

https://doi.org/10.4077/CJP.2012.BAA061

並列摘要

Dysregulation of epidermal growth factor receptor (EGFR) has been associated with colorectal cancer, but no evidence shows a relationship of EGFR expression to clinicopathological parameters in colorectal cancer in Taiwan. Immunohistochemical analysis of EGFR, Ki67, and p53 was performed in tissue microarray slides of 9 normal glandular tissues and 150 specimens including 49 well, 58 moderately, and 43 poorly differentiated colorectal adenocarcinomas. Differences in intensity of EGFR immunostaining and the percentages of Ki67 and p53 positive cells between normal and tumor specimens were evaluated using the Student t-test, one-way ANOVA, and linear regression analysis. Intensity of EGFR staining was weak and nuclear expression of Ki67 and p53 was scattered in all 9 control specimens. Expression of Ki67 and p53 but not EGFR was significantly higher in more advanced grades and TNM stages of colorectal adenocarcinoma than in normal controls. The percentages of Ki67 and p53 stained tumor cells were significantly higher in moderately (Ki67: 60.3 ± 6.5, p53: 47.6 ± 3.8) and poorly (Ki67: 61.8 ± 5.3, p53: 55.1 ± 4.1) differentiated tumor cells than in well differentiated (Ki67: 40.8 ± 4.4, p53: 39.8 ± 4.2) tumor cells. Additionally, the Ki67 and p53 staining intensity was also significantly correlated with the more advanced T, N and American Joint Committee on Cancer (AJCC) clinical stage of colorectal adenocarcinoma, suggesting their usefulness as biomarkers of colorectal adenocarcinoma progression. In conclusion, EGFR immunochemistry may not be a good method for pre-treatment evaluation of colorectal adenocarcinoma in Taiwan.

並列關鍵字

colorectal adenocarcinoma ； EGFR ； Ki67 ； p53 ； tissue microarray

參考文獻

Aimes, R. T.,Zijlstra, A.,Hooper, J. D.,Ogbourne, S. M.,Sit, M. L.,Fuchs, S.,Gotley, D. C.,Quigley, J. P.,Antalis, T. M.(2003).Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis.Thromb. Hemost..89,561-572.

Allegra, C. J.,Paik, S.,Colangelo, L. H.,Parr, A. L.,Kirsch, I.,Kim, G.,Klein, P.,Johnston, P. G.,Wolmark, N.,Wieand, H. S.(2003).Prognostic value of thymidylate synthase, Ki67, and p53 in patients with Dukes B and C colon cancer: a national cancer institute-national surgical adjuvant breast and bowel project collaborative study.J. Clin. Oncol..21,241-250.

Arber, N.,Hibshoosh, H.,Yasui, W.,Neugut, A.I.,Hibshoosh, A.,Yao, Y.,Sgambato, A.,Yamamoto, H.,Shapira, I.,Rosenman, D.,Fabian, I.,Weinstein, I. B.,Tahara, E.,Holt, P. R.(1999).Abnormalities in the expression of cell cycle-related proteins in tumors of the small bowel.Cancer Epiderm. Biomar..8,1101-1105.

Benaud, C.,Dickson, R. B.,Lin, C.Y.(2001).Regulation of the activity of matriptase on epithelial cell surfaces by a blood-derived factor.Eur. J. Biochem..268,1439-1447.

Benaud, C. M.,Oberst, M.,Dictson, R. B.,Lin, C. Y.(2002).Deregulated activation of matriptase in breast cancer cells.Clin. Exp. Metastasis..19,639-649.