- 期刊
Hot-pressurized Fluid Extraction of Flavonoids and Phenolic Acids from Brazilian Propolis and Their Cytotoxic Assay in vitro
熱壓水溶性巴西蜂膠及體外細胞生化試驗
摘要
本研究成功開發熱壓水乳化之不溶性蜂膠的程序，並探討水溶及脂溶蜂膠萃出液的抗氧化與抑制癌細胞的能力。分別進行常壓乙醇與水萃取程序，及熱壓乙醇與水萃取程序實驗的數據比較。結果顯示，熱壓水溶性蜂膠濃度，可逹35.2mg/mL，較無熱壓乳化的樣品高出44%。在清除DPPH自由基的能力方面，熱壓水溶性蜂膠樣品，均具有顯著的抗氧化功能，其有效濃度EC50值，可低至16μg/mL。根據血癌、肺癌、肝癌三種癌細胞的體外生物活性測試數據，顯示蜂膠萃出液之抑制六種癌細胞的存活率，皆具有濃度的相依性。熱壓水溶蜂膠萃出液，對於血癌細胞HL-60之有效濃度EC50值，甚至低於11μg/ML。
關鍵字
無資料並列摘要
This work has examined the hot-pressurized fluid extraction of seven flavornoids, caffeic acid phenethyl ester and four phenolic acids from Brazilian propolis lumps generating, during the process, fat-and water-soluble extracts. The solid content of water-soluble extract obtained by hot-pressurized water in the presence of 29% natural surfacetant was 35.2mg/mL and was 44% greater than that obtained without natural surfacetant. Furthermore the amount of the seven flavornoids and caffeic acid phenethyl ester in the fat-soluble extract exceeded those in the water-soluble sample while, on the other hand, the amount of the four phenolic acids in the water-soluble extract was more than those in the fat-soluble extract. Our findings show that the total solid content and the amount of these 12 active compounds produced by the emulsitied hot-pressurized water are 36% and 7% higher, respectively, than those produced by emulsified water at atmospheric pressure. The EC50 value of the free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl of the emulsified hot-pressurized water extract was the lowest, and presented the strongest anti-oxidation ability among all the extracts. In vitro cytotoxicity indicated that the water-soluble extract strongly suppressed the growth of leukemia (HL-60, U937), lung cancer(A549, CH27) and liver cancer (Hep G2, Hep 3B) cell in a concentration-dependent behavior.