- 期刊
- OpenAccess
Muscle Development in Larvae of the Red Sea Bream Pagrus Major
嘉鱲魚仔魚時期肌肉的發育
摘要
The present experiment investigated the effects of aquatic bacteria,Pseudomonassp.,on the water quality and bacterial flora in hatching ponds of giant tiger prawn(Penaeusmonodon).In giant tiger prawn larval rearing waters where bactericides have not beenapplied,the count of colony forming units in 1 ml of rearing water(CFU/ml)may reach arange of 4.6×10^(4)～2.56×10^(12).Addition of a suspension of viable.Pseudomonas sp.to thisrearing water did not affect the total viable bacterial count(P＞0.05).However,Pseudomonassp.was able to inhibit the growth of Vibrio in the rearing waters since its quantityin the two experimental ponds was lower than that in the control.Prior to hatchery operation,the bacterial genera detected in the experimental pondwater were metabolically inactive species(e.g.Acinetobacter,Moraxella and Kingella).Active strains(e.g.Aeromonas,Haemophilus,Salmonella,and Vibrio)dominated the bacterialflora when artificial feed was introduced.In the case of the occurrence of mass mortality,the majority of metabolically active species that existed in the rearing water were thepathogenic bacteria V.parahaemolyticus and V.alginolyticus.From PL4 to PL9 stages,when Pseudomonas sp.suspension of 300 ml/day(1.0×10^(9)CFU/ml was added into the rearing water,the unionic ammonia-N(NH3-N)concentrationwas lower in the experimental ponds than in the control.Furthermore,the concentration ofNH3-N in the experimental ponds did not affect the survival rate(r=1:-0.533 & 1:-0.5742),but the situation appeared to be exactly opposite in case of the control(r=1:-0.926).Asignificant difference was hence observed from the M3 to PL9 between the survival ratesof prawn larvae in treated and control ponds(P＜0.05).
並列摘要
The present experiment investigated the effects of aquatic bacteria,Pseudomonassp.,on the water quality and bacterial flora in hatching ponds of giant tiger prawn(Penaeusmonodon).In giant tiger prawn larval rearing waters where bactericides have not beenapplied,the count of colony forming units in 1 ml of rearing water(CFU/ml)may reach arange of 4.6×10^(4)～2.56×10^(12).Addition of a suspension of viable.Pseudomonas sp.to thisrearing water did not affect the total viable bacterial count(P＞0.05).However,Pseudomonassp.was able to inhibit the growth of Vibrio in the rearing waters since its quantityin the two experimental ponds was lower than that in the control.Prior to hatchery operation,the bacterial genera detected in the experimental pondwater were metabolically inactive species(e.g.Acinetobacter,Moraxella and Kingella).Active strains(e.g.Aeromonas,Haemophilus,Salmonella,and Vibrio)dominated the bacterialflora when artificial feed was introduced.In the case of the occurrence of mass mortality,the majority of metabolically active species that existed in the rearing water were thepathogenic bacteria V.parahaemolyticus and V.alginolyticus.From PL4 to PL9 stages,when Pseudomonas sp.suspension of 300 ml/day(1.0×10^(9)CFU/ml was added into the rearing water,the unionic ammonia-N(NH3-N)concentrationwas lower in the experimental ponds than in the control.Furthermore,the concentration ofNH3-N in the experimental ponds did not affect the survival rate(r=1:-0.533 & 1:-0.5742),but the situation appeared to be exactly opposite in case of the control(r=1:-0.926).Asignificant difference was hence observed from the M3 to PL9 between the survival ratesof prawn larvae in treated and control ponds(P＜0.05).