Structural Changes in the Muscular Tissue of Thornfish (Terapon jarbua, Forsskål) under TBT (Tributyltin) Exposure

三丁基錫對花身雞魚軀幹肌之慢性毒性作用

王達益(Dar-Yi Wang) ； 黃寶貴(Bao-Quey Huang) ； 翁進坪(Jinn-Pyng Ueng)

《臺灣水產學會刊》 Volume 31 Issue 3 (2004/09) Pp. 225-234

https://doi.org/10.29822/JFST.200409.0006

Abstracts

In order to study the toxic effects of an antifouling agent, tributyltin (TBT), thornfish (Terapon jarbua Forsskål) were exposed to 1 and 5μg/l TBT for 60 days and the morphological changes of the trunk muscle in this species were examined. Light microscopical examination revealed severe muscle atrophy and/or necrosis in the 1 and 5μg/l TBT group, vacuolation among myofibrils and the broken myofibrils in the injured white muscular tissue. The morphometric analysis showed that the relative area of vacuolation was dosage-dependent. Swelling of mitochondria and cristae degeneration were detected from injured muscle cells of the 5μg/l TBT-treated fish using a transmission electron microscope (TEM). Additionally, the relative body height significantly decreased from 30.8±0.7% (control group) to 28.0±0.8% (1μg/l TBT group) and 27.6±0.9% (5μg/l TBT group) (p＜0.05, n=10), revealing slimmer and/or thinner body shape. This study elucidated histopathologic diagnoses of muscle disorders due to TBT toxicity.

Keywords

抗生物附著劑 ； 三丁基錫 ； 肌肉形態 ； 形態數值分析 ； 粒線體腫脹

Parallel abstracts

In order to study the toxic effects of an antifouling agent, tributyltin (TBT), thornfish (Terapon jarbua Forsskål) were exposed to 1 and 5μg/l TBT for 60 days and the morphological changes of the trunk muscle in this species were examined. Light microscopical examination revealed severe muscle atrophy and/or necrosis in the 1 and 5μg/l TBT group, vacuolation among myofibrils and the broken myofibrils in the injured white muscular tissue. The morphometric analysis showed that the relative area of vacuolation was dosage-dependent. Swelling of mitochondria and cristae degeneration were detected from injured muscle cells of the 5μg/l TBT-treated fish using a transmission electron microscope (TEM). Additionally, the relative body height significantly decreased from 30.8±0.7% (control group) to 28.0±0.8% (1μg/l TBT group) and 27.6±0.9% (5μg/l TBT group) (p＜0.05, n=10), revealing slimmer and/or thinner body shape. This study elucidated histopathologic diagnoses of muscle disorders due to TBT toxicity.

Parallel keywords

Antifoulant ； Tributyltin ； Muscle injury ； Histopathologic ； Morphometric analysis