本研究探討在密閉包裝環境下,分別以包括丁香油、丁香酚、異丁香酚、苯唑卡因、二苯氧基乙醇、哪啶及三卡因甲磺酸等不同麻醉劑添加於包裝水體,對大和米蝦進行72小時模擬運輸後之麻醉效果與活存率差異。包括丁香油、丁香酚與異丁香酚之丁香醚麻醉劑、苯唑卡因、二苯氧基乙醇、哪啶及三卡因甲磺酸分別於濃度達25, 200, 600, 100及100 μl/l對大和米蝦具麻醉效果。丁香油、丁香酚與異丁香酚對大和米蝦48小時之半致死濃度分別為41.53, 35.14及27.44 μl/l。模擬運輸下添加丁香醚麻醉劑,可有效降低總氨排泄並減緩運輸過程pH變化。比較3種丁香醚麻醉劑應用於大和米蝦模擬運輸,添加丁香酚之組別較丁香油及異丁香酚具相對較高活存率。而添加丁香醚麻醉劑皆可延遲包裝水體pH之上升與氨排泄累積速率。
This study was investigated the survival and anesthesis of a 72-h simulated transport of Amano shrimp Caridina multidentata enclosed in packing bags with water containing seven anesthetics: clove ether-containings (clove oil, eugenol and isoeugenol), benzocaine, 2-phenoxyethnaol (2-PE), quinaldine, and tricaine methanesulfonate (MS-222). Clove ether-containings, benzocaine, 2-PE, quinaldine, and MS- 222 at doses of 25, 200, 600, 100, and 100 μl/l caused sedative and anesthetic effects on Amano shrimp, respectively. The 48-h LC50 (median lethal concentration) of clove oil, eugenol and isoeugenol of shrimp was 41.53, 35.14, and 27.44 μl/l, respectively. Among the anesthesia during simulated transport, clove ether-containing effectively reduced ammonia excretion, and pH just a little varied in the water containing clove oil, eugenol and isoeugenol compared with control. The shrimp exposed to eugenol has higher survival than those exposed to clove oil and isoeuegnol during simulated transport. Addition of clove ethercontaining delayed the increase in pH and reduced the ammonia excretion of shrimp.