Evaluation of hybridization and spawning ground between Amphiprion ocellaris and Amphiprion ocellaris var.

The main objective of this study was to develop clownfish with specific body colors through hybridization experiments with Amphiprion ocellaris (female) and A. ocellaris var. (male). The experiment also investigated artificial pairing behavior, choice of artificial ovaries, spawning rates, and juvenile morphology. Four types of artificial spawning substrates-smooth tiles (A), rough tiles (B), ceramic flower pots (C), and plastic PVC pipes (P)-were placed in breeding tanks. A total of 12 pairs of broodstock were observed over a period of 16-months to monitor pairing behavior, spawning conditions, and willingness to choose spawning grounds. The results showed that B and C had a higher number of eggs, whereas no spawning was observed in A and P. The average number of eggs laid was 303 ± 0.27 on B and 332 ± 0.57 on C, with no significant difference between the two. These findings suggest that rough tiles and ceramic pots are more suitable spawning substrates than smooth tiles and PVC pipes for A. ocellaris breeding under artificial conditions. The body color of the juvenile fish is different from that of the parents. It has an orange-red chest, a brown body, and a three-white stripe pattern.

Keywords

hybridization ； spawning ground ； spawning ； Amphiprion ocellaris

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