The purpose of the experiment was to investigate the relationship between water uptake and osmotic potential of the pedicel structures in Eustoma cut flower in the vase. The water uptake ability of pedicels of 'Shuang-Bair-Tzyy' cultivars was higher than 'Shuang-Liuh' in the beginning 9 days. The water uptake content of 'Shuang-Bair-Tzyy' cultivars reached up to its maxi, 0.8ml, on the 1st vase day. The osmotic potentials of pedicels of both cultivars have increased during the beginning 2 days, but 'Shuang-Bair-Tzyy' cultivars sustained lower osmotic potential in the beginning 10 days. The cells of pedicel tissue were turgid before vase stage and bad max. water uptake stage. 'Shuang-Liuh' had higher cell wilting percentage than 'Shuang-Bair-Tzyy' at the silting stage. The cells of phloem and cambium tissue wilted seriously but vessel cells remained unchanged in both cultivars at the wilting stage. The results showed that lower osmotic potential would keep the water uptake ability higher and persist water holding of pedicel tissue during the vase days.