Different soils might have various effects on the function of plant growth-promoting microorganisms (PGPMs) which could promote plant growth and foliar chemical contents. However, the interaction effects between soil types and PGPMs have not been studied. In this study, two soil types (field soil and commercial growth medium) treated with or without PGPMs (a fungus and mixed bacterial inoculants) were used to study their effects on cabbage plants. Plant growth (dry weight and leaf area), foliar nutrient contents (water and protein content), and foliar antinutrient chemicals (polyphenol oxidase and trypsin inhibitor) were also analyzed. Moreover, the foliage collected from different treatments was also fed to the third instar larvae of Spodoptera litura. The result showed that PGPMs significantly increased plant growth. Moreover, the commercial growth medium treated with PGPMs produced the highest biomass. The interaction effect between PGPMs and soil types was significant for the water content; whereas the anti-nutrient compound was affected by the soil type only. Finally, a significant combined soil type and PGPM effect was observed for the third instar performance of S. litura. In summary, our study suggests that the function of PGPMs might be affected by soil type. PGPMs would perform best when inoculated in the commercial growth medium which contains suitable nutrients. Therefore, the future use of PGPMs should also consider the soil nutrient status before application to obtain their appropriate efficacy.