Enzymatic Synthesis of β-Imidazolyl-4(or 5)-Pyruvic Acid by Rat Liver Histidine-Pyruvate Aminnotransferase

應用鼠肝組織胺酸－丙酮酸轉胺酶合成咪唑丙酮酸

尹士俊(Shih-Jiun Yin) ； 李詩慶(Shih-Ching Lee)

《Journal of Medical Sciences》 1卷4期 (1976/12) Pp. 331-340

摘要

應用部分純化之大白鼠肝組織胺酸－丙酮酸轉胺酶，嘗試一種新的合成咪唑丙酮酸途徑的可能性。大白鼠接受皮下注射升血糖激素三天後，肝組織胺酸－丙酮酸轉胺酶活性增加十四倍。經加熱處理及硫酸銨沉澱法將肝組織胺酸－丙酮酸轉胺酶部分純化，以EDTA為組織胺酸去氨酶之抑制劑，進行由組織胺酸合成咪唑丙酮酸。最終之合成萃取液經纖維素薄層色層分析和胺基酸自動分析儀的分析結果，均顯示所述合成步驟的可行性。應用低溫結晶法並可獲得咪唑丙酮酸的結晶。

並列摘要

A new approach to synthesize β-Imdazolyl-4 (or 5)-pyruvic acid through partially purified rat liver histidine-pyruvate aminotransferase was investigated. Histidine-pyruvate aminotransferase activity could be increased 14-fold in rats injected subcutaneously with glucagon for 3 days. The liver enzyme was partially purified by heat treatment and ammonium sulfate fractionation. EDTA was used as the histidase inhibitor for the liver enzyme preparation. Imidazolepyruvic acid was then synthesized from histidine by using the partially purified aminotransferase. Thin-layer chromatographic analysis and amino acid analysis of the final extract indicated the feasibility of the described synthetic processes. Crystals of imidazolepyruvic acid were also obtained by low temperature crystallization.