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Sparteine刺激離體子宮收縮之機轉
Mechanism of in Vitro Uterine Contraction Induced by Sparteine in the Rat
摘要
以雌性大白鼠為實驗動物，分為兩組，第一組是未先經indomethacin處理過的，將其子宮取出，於離體狀態下，記錄其收縮，觀察sparteine、oxytocin，及前列腺素（prostaglandin）刺激子宮收縮的情形，以及加入不同濃度的indomethacin以後，對於它們作用的影響。發現indomethacin隨劑量的增大（0.01~0.1mM）對sparteine及oxytocin作用的抑制也愈強，而對於前列腺素E2（0.1~0.5μM）刺激子宮的作用則無抑制作用。另一組實驗是用先經indomethacin處理的雌鼠為對象，即在實驗前48，24及1小時，連續三次肌肉注射10mg/kg的indomethacin。在此劑量下，大白鼠體內前列腺素的合成與釋放均受到抑制。取出這一組動物的子宮作離體實驗，則發現其子宮肌肉對sparteine，oxytocin的反應均降低，但對PGE2或PGF2α的反應則仍很好。由以上結果推論，sparteine刺激大白鼠子宮收縮的作用，可以主要是經由促進前列腺素釋放造成的。
關鍵字
無資料並列摘要
The in vitro responses of rat uterine muscle to sparteine, and prostaglandin E2 or F2α were studied. All of these agents induced uterine market contraction. Indomethacin (0.01-0.1mM) inhibited the stimulatory effect of sparteine and oxytocin, but did not affect the effect of prostaglanding E2 or F2α. In estadiol pretreated rats, administration (i.m.) of indomethacin systematically reduced the initial uterine motility and reactivity to sparteine and oxytocin, but did not alter the response of PGE2 or PGF2α. Since indomethacin inhibits the prostaglandins synthesis. Our results suggest that the action of sparteine is largely mediated through the release of prostaglandins from the uterine muscle.