Neutrophils and Chemiluminescence-The Effect of Phorbol Myristate Acetate (PMA) and Oxygen Radicals Scavengers

中性白血球及冷光－白血球刺激劑PMA及氧游離基吞吃劑的作用

汪大衛(David Wang) ； 徐剛(Kang Hsu) ； 沈建業(Chien-Yeh Shen) ； 謝善德(Shang-Der Hsieh)

《Journal of Medical Sciences》 7卷2期 (1986/09) Pp. 75-80

摘要

本研究中我們發現PMA所激活的白血球可產生冷光，而氧游離基吞吃劑（過氧游離基轉化酶或觸酶）對冷光之產生有與其劑量呈正比關係的抑制作用。過氧游離基轉化酶在625μg/ml的濃度下可發揮95%的抑制，而觸酶在3mg/ml的濃度下發揮70%的抑制。我們的結果認為PMA所激活的白血球可放出許多氧游離基，藉此可解釋PMA可產生實驗性急性呼吸窘迫徵候群以及何以氧游離基吞吃劑可減輕其徵候群的道理。

關鍵字

肺損傷 ； 氧游離基吞吃劑 ； 冷光 ； 中性白血球

並列摘要

When Phorbol Myristate Acetate (PMA) was used to activate neutrophils it was found that the activated neutrophils could produce chemiluminescence (CL), whereas oxygen radicals scavengers, superoxide dismutase (SOD) or catalase showed a dose-dependent inhibition of CL production. SOD at 625 μg/ml concentration produced 95% inhibition; catalase at 3 mg/ml concentration produced 70% inhibition. These results indicated that the PMA-activated neutrophils may enhance the release of oxygen radicals. Further, the results might also explain why PMA omit causes experimental adult respiratory distress syndrome (ARDS) and why SOD and catalase omit attenuate lung damage in several ARDS models.

並列關鍵字

Lung damage ； Phorbol myristate acetate ； PMA ； Neutrophil ； Chemiluminescence ； Oxygen radicals scavengers