- 期刊
- OpenAccess
裸屬之育種繁殖及飼養
Breeding, Reproduction and Raising of Nude Mice
摘要
The nude mouse is a mutant species. Because nude mice inherently 1ack the thymus, we can take advantage of this immune defect to transp1ant human cancer or to conduct infection experiments that impossib1e to carry out on other anima1s. Our nude mice 1aboratory was first estab1ished in 1977, having accumu1ated 13 years of experience in their breeding, reproduction and raising. The surviva1 rate of our nude mice six months after birth has risen from 12 % to 90 %. 1n the initial stage, we needed to add high doses of antibiotics (ampicillin 500 mg / 1, tetracycline 750 mg / 1) and vitamins to their drinking water. At present, owing to improvements in environment, we on1y use formalin to smoke the rooms and weekly use gun fire and 0.1 % benzalkonium chloride solution to sterilize the environments. 1n addition, we regularly conduct bacterial colony tests of their evnironment and cultures of their excreta to assure a specific pathogen-free condition. In order to obtain the best results in newborn number, weaned rate and survival rate, we compared four different methods of mating. The first method was to use female and male nude mice. Because female nudes were poor nurses having a tendency tend to eat their newborn and the weaned rate was only 32 - 34 %, thus the results were not satisfactory. The intercourse between female nudes and male haired mice with nude gene (second method) resulted in low rate of newborn nudes (47-52%) and low weaned rate (31-33%). The third method involving mating female and male haired mice with nude gene produced high weaned rate (89 - 93 %). However, the rate of obtaining newborn nudes was very low (17 - 23%). The best method was mating the haired females with nude gene to male nudes. Although this method produced lower rate of newborn nudes (47 -48%) than the mating of pure male and female nudes (100%), the weaned rate was as high as 93-94 %. Therefore, we recommend the use of the fourth method. If newborn nudes found to be weak and thin, we added artificial nutrition consisting of subcutaneous glucose (5 %) and oral poly vitamins so as to attain a higher weaned rate up to 98 %. The number of nude mice reproduced per year in our laboratory reached as high as 5000, thereby enabling us to supply researchers throughout the country.
並列摘要
The nude mouse is a mutant species. Because nude mice inherently 1ack the thymus, we can take advantage of this immune defect to transp1ant human cancer or to conduct infection experiments that impossib1e to carry out on other anima1s. Our nude mice 1aboratory was first estab1ished in 1977, having accumu1ated 13 years of experience in their breeding, reproduction and raising. The surviva1 rate of our nude mice six months after birth has risen from 12 % to 90 %. 1n the initial stage, we needed to add high doses of antibiotics (ampicillin 500 mg / 1, tetracycline 750 mg / 1) and vitamins to their drinking water. At present, owing to improvements in environment, we on1y use formalin to smoke the rooms and weekly use gun fire and 0.1 % benzalkonium chloride solution to sterilize the environments. 1n addition, we regularly conduct bacterial colony tests of their evnironment and cultures of their excreta to assure a specific pathogen-free condition. In order to obtain the best results in newborn number, weaned rate and survival rate, we compared four different methods of mating. The first method was to use female and male nude mice. Because female nudes were poor nurses having a tendency tend to eat their newborn and the weaned rate was only 32 - 34 %, thus the results were not satisfactory. The intercourse between female nudes and male haired mice with nude gene (second method) resulted in low rate of newborn nudes (47-52%) and low weaned rate (31-33%). The third method involving mating female and male haired mice with nude gene produced high weaned rate (89 - 93 %). However, the rate of obtaining newborn nudes was very low (17 - 23%). The best method was mating the haired females with nude gene to male nudes. Although this method produced lower rate of newborn nudes (47 -48%) than the mating of pure male and female nudes (100%), the weaned rate was as high as 93-94 %. Therefore, we recommend the use of the fourth method. If newborn nudes found to be weak and thin, we added artificial nutrition consisting of subcutaneous glucose (5 %) and oral poly vitamins so as to attain a higher weaned rate up to 98 %. The number of nude mice reproduced per year in our laboratory reached as high as 5000, thereby enabling us to supply researchers throughout the country.