- 期刊
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Peripheral Blood Mononuclear Cell Collection and Processing
周邊血單核細胞之收集及處理
摘要
為替6位患有血液腫瘤之病患收集周邊幹細胞以後救援之用，以白血球分離術收集其單核球。病患於緩解期時，分別以COBE 2997及Fenwal CS300細胞分離儀作23及2次之白血球分離術。收集得之單核球量為(3±1.04 ) ×l0^9 （平均值±標準差）。經處理後為(2.5±0.8) ×10^9，耗損率為25%。若以COBE 2997操作，所得成份之紅血球比容為(1.4±0.69) %，血小板污染數為138×10^3至1584×10^3/微升。收集得之褐黃層懸浮於10% DMSO與血漿之混合液中，以程式冷凍至-90℃後眝存於液態氮桶中。發現所收集得之單核球數與病人血液中之單核球讀數間之相關性未達統計上之意義。為評估收集及處理之效果，尚有待輸注幹細胞後之臨床追蹤及幹細胞培養。
並列摘要
In order to obtain peripheral blood stem cells for bone marrow rescue in the future, mononuclear cells were collected by means of leukapheresis for 6 patients with hematological malignancies. Twenty three procedures were performed by the cell separator COBE 2997 and two by Fenwal CS-3000 cell separator. The patients were in the remission stages during leukapheresis. The amount of mononuclear cells was (3 ± 1.04) × 10^9 (mean ± SD). The cell number was (2.5 ± 0.8) × 10^9 after processing, with 25% loss. By COBE 2997, the hematocrit of the products was (1.4 ± 0.69)% and the contaminated platelets were ranged from 138 × l0^3 to 1584 × 10^3 /μl. The buffy coat was suspended in 10% dimethyl sulfoxide (DMSO)-plasma, and was stored in the liquid nitrogen tank after being frozen to -90°C with the programmed freezer. The number of mononuclear cells collected was not statistically correlated with the patients' blood mononuclear cell count. Clinical follow up of the patients after reinfusion and the stem cell culture of the buffy coat products may be helpful to assess the effectiveness of the collecting and processing procedures.