- 期刊
不孕症病人使用誘導排卵藥物前後其血清中MMP-2與MMP-9暨E2及CA125之變化分析
Analysis of the MMP-2, MMP-9 Activity, E2 and CA125 Level Before and after hCG Treatment on Infertility Women
摘要
In clinical practice, evaluating the follicle maturity tends to use ultrasound monitoring the number and size of follicles as well as check the blood E2 level. The aim of this study is to find out the changes of MMP-2、MMP-9、CA-125 and E2 in infertile women after ovarian induction and the correlation of these molecules with follicle number. We collected serum samples before and after hCG injection. The MMP-2 and MMP-9 protein were analyzed by gelatin zymography and the concentrations of CA-125 and E2 were determined by microparticle enzyme immunoassay. The results indicated that the levels of MMP-2 and CA-125 were not significantly changed after hCG injection, but the amount of MMP-9 was significantly increased after hCG injection in patients who had 5 or more follicles (p＜0.05) and was significantly decreased in patients with 1~4 follicles (p＜0.05). The concentration of E2 also has significantly decreased after hCG injection (p＜0.05). Among 76 patients, 11 were pregnant successfully and their CA-125 value were higher than that of non-pregnant women by 11.3%. In conclusion, it appears that there is a correlation between follicle number and MMP-9. In the future, we will include both follicle fluid and serum into analysis to build another set of reference data for estimating the maturity follicle and oocyte.
並列摘要
In clinical practice, evaluating the follicle maturity tends to use ultrasound monitoring the number and size of follicles as well as check the blood E2 level. The aim of this study is to find out the changes of MMP-2、MMP-9、CA-125 and E2 in infertile women after ovarian induction and the correlation of these molecules with follicle number. We collected serum samples before and after hCG injection. The MMP-2 and MMP-9 protein were analyzed by gelatin zymography and the concentrations of CA-125 and E2 were determined by microparticle enzyme immunoassay. The results indicated that the levels of MMP-2 and CA-125 were not significantly changed after hCG injection, but the amount of MMP-9 was significantly increased after hCG injection in patients who had 5 or more follicles (p＜0.05) and was significantly decreased in patients with 1~4 follicles (p＜0.05). The concentration of E2 also has significantly decreased after hCG injection (p＜0.05). Among 76 patients, 11 were pregnant successfully and their CA-125 value were higher than that of non-pregnant women by 11.3%. In conclusion, it appears that there is a correlation between follicle number and MMP-9. In the future, we will include both follicle fluid and serum into analysis to build another set of reference data for estimating the maturity follicle and oocyte.