- 期刊
以多位基因座序列分析法和脈衝式電泳分析法比較嗜肺性退伍軍人菌分子分型
Compare Molecule Typing Method with Pulsed-field Gel Electrophoresis and Multilocus Sequence Typing in Legionella Pneumophila
摘要
退伍軍人症(Legionnaire disease; LD)是由退伍軍人菌，尤其是嗜肺性退伍軍人菌血清型第一型(Legionella pneumophila serogroup 1; Lp 1)感染引發肺炎的疾病。台灣自1995年起，退伍軍人症確定病例每年平均爲69例。本研究將2001-2004年來收集之47株臨床菌株和環境菌株，分別以多位基因座序列分析法(multilocus sequence typing, MLST)和脈衝式電泳分析法(pulsed-field gel electrophoresis, PFGE)分析並比較其差異。在MLST分型結果發現，不同血清型Lp均有不同型別，而以M1爲優勢型別(34.1%)，另外，有9株爲新型別(20.9%)。18株Lp臨床株中，新型占5株(27.8%)，25株Lp環境株中，新型占3株(12%)，表示台灣退伍軍人菌有台灣特有的菌株型別，比例高達20%，且臨床株的多樣性比環境株還大。PFGE分型結果可分成4區，D區均爲L.dumoffii。由PFGE親緣樹狀圖譜可知，環境株中DNA片段最多僅相差4個片段，而臨床株最多相差10個DNA片段，顯示臨床株基因體DNA序列差異比環境株大，此結果與MLST分型結果相似。MLST方法分型率爲47.7%；PFGE方法分型率爲81.3%。
並列摘要
Objective: Legionnaire disease (LD) is caused by Legionella, especially the Legionella pneumophila serogroup 1 (Lp 1). In Taiwan, the case number reported for each year was about 69 since the year 1995. In this study, a total of 47 clinical and environmental strains collected from 2001 to 2004 were used for typing studies. The pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) methods were performed. In MLST results, Lp strains with different serogroups showed different genotypes, with the dominant type being M1 (34.1%). Moreover, 9 strains with new types were reported, expressing that in Taiwan Lp has unique variant strains, and the proportion percentage was as high as 20%. Comparatively, clinical strain variations are larger than environment strains. In PFGE results, strains were grouped into 4 clusters, with D cluster mainly being L. dumoffii. Based on the results from PFGE analysis, the DNA fragments from environmental strains mostly showed differences in only 4 fragments, compared with clinical strains with differences up to 10 fragments. In conclusion, clinical strains showed a higher genetic variation than the environment strains. This PFGE and MLST results were similar, with MLST typing rate being 47.7%, and PFGE typing rate 81.3%.