- 期刊
Syva Emit d.a.u. 和Emit st安非他命試劑的比較
Comparison of the Syva Emit d.a.u. and Emit st Amphetamine Assay Reagents
摘要
本研究旨在比較Syva公司所生產的三種安非他命篩檢試劑，這三種產品分別是Emit d.a.u. 多株抗體，Emit d.a.u. 單株抗體和Emit st多株抗體試劑，由於配方和標準液中所含安非他命種類及濃度的不同，所得到的結果也不一樣。在花蓮地區檢警單位送檢的檢體中，使用Emit d.a.u. 多株抗體安非他命試劑和d.a.u. 鴉片類試劑來測試，所得的陽性反應應盛行率如下：安非他命79%，鴉片類11%，兩者都有者佔7%，陰性反應者只佔17%，使用d.a.u. 試劑，應用於不同的儀器，如Hitachi 747和Molecular Devices的Microplate Reader，都能得到滿意的結果。
並列摘要
There are three different Syva Emit amphetamine assay reagents currently in use in Taiwan, namely: Emit st amphetamine, Emit d.a.u. amphetamine (polyclonal antibody, cutoff 300 ng/ml) and Emit d.a.u. amphetamine/methamphetamine (monoclonal, cutoff 1000 ng/ml). The Emit st reagent is easy to perform, does not require expensive instrument, the personnel does not require extensive training. However, it is inefficient (30 samples per hour) and expensive (260 NT dollars per sample). The Emit d.a.u. polyclonal and monoclonal amphetamine assays can be adapted to automated clinical chemistry analyzer (Hitachi 747). It is very efficient (600 samples per hour, maximum 2400 tests per hour). It wastes about 5 ml of working reagents to prime the instrument. We compared the performance of the Emit st and Emit d.a.u. polyclonal amphetamine reagents and found that these two reagents are comparable. The d.a.u. assays cost only about one tenth of the st reagents. The monoclonal d.a.u. amphetamine assays are more specific, but the separation between the calibrator and the negative sample is smaller than the polyclonal reagent. The d.au. reagents can also be adapted to the microplate reader from the Molecular Devices. Assays performed with plates reader require manual addition of samples and reagents.