- 期刊
Comparison of the Infectivity in Filamentous and Spherical Influenza Virus
長條型及圓球型流行性感冒病毒之感染力比較研究
摘要
流行性感冒病毒，除了常見的圓球型外，長條型感冒病毒也曾被報告見之於各主要亞型的流行性感冒病毒，特別是新分離出之病毒。長條型病毒具有各種圓球型病毒之生物特性，然而其感染力至今仍無定論。本研究以兩A株流行性感冒病毒，A/WSA/33及NWS-F，來自於相同的母株，僅M基因及NA基因不同，分別代表圓球型及長條型病毒。其形態以電子顯微鏡觀察，其感染力以斑點檢定法測定，病毒顆粒數量以凝血檢定法估計，所有的研究者在研究過程中均不知道病毒之識別，並且病毒之形態均經Dr. E.D Kilbourne之實驗室雙重確認，以避免誤差。研究結果顯示長條型流行性感冒病毒在MDCK細胞具有較強的感染力，雖然流行性感冒病毒在人體上並無直接證據顯示以長條型態存在，但長條型流行性感冒病毒在流行感染上可能有其重要性。
並列摘要
In addition to spherical influenza virus, filamentous virus particles have been noted in every main strains of influenza virus examined, especially in recently isolated strains. Although the filamentous influenza virion has been found to possess many of the biological properties of spherical influenza virus, the question of the infectivity of filaments relative to that of spheres remains in doubt. Two influenza A virus, A/WSN/33 and NWS-F, representing spherical and filamentous morphology, respectively, were supplied by professor E.D. Kilbourne. A/WSN/33 and NWS-F came from same parent strain but NWS-F containing the M gene and NA gene of RI/5+, which has high proportion of filamentous particles. The morphology of the viruses were revealed under the electron microscope. The infectivity were estimated by plaque assay and the quantity of virus particle was determined by hemagglutination assay. To avoid prejudice, the identities of the viruses have been kept blind to researchers until the morphological feature and infectivity of the viruses had been determined. The results indicated that the influenza filaments have higher infectivity in MDCK cells than that of the spheres. Although the presence of filaments has not yet been demonstrated in the nasal washing from infected human, it may be of practical importance in the monitoring of influenza epidemics.