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  • Journals

No Association of the S20g Mutation of Amylin Gene with Insulin Secretion, Insulin Sensitivity, and Diabetes Mellitus in Taiwanese

Parallel abstracts


To study the role of the S20G mutation of amylin gene in the pathogenesis of type 2 diabetes, 99 unrelated normal controls, 24 subjects with impaired glucose tolerance, 182 subjects with MIDDM, and 122 subjects with IDDM were recruited in Taiwan. The S20G mutation of amylin gene was detected by polymerase-chain-reaction/restriction-fragment-length-polymorphism and confirmed by DNA sequence analyses. Pedigree members of the families with this mutation were studied by oral glucose tolerance test, glucagon stimulation test, and insulin suppression test. The S20G mutation of the amylin gene was found in 4 (4.4%) in normal control subjects, 1 (4.2%) in 24 subjects with IGT, 2 (1.6%) in subjects with type 1 diabetes, and 3 (1.6%) in the subjects with type 2 diabetes. Among the non-diabetic pedigree members, subjects with mutation had a reduced response of insulin secretion at 30 min and an increase in plasma glucose concentration at 60 min after oral glucose loading as compared to those without mutation. The maximal insulin secretion was delayed in the subjects with mutations. However, these differences were not found after adjustment with age factor. No difference in the steady-state plasma glucose levels was found between the non-diabetic subjects with or without mutation. In conclusion, S20G mutation of amylin gene does not seem to play a major role in the pathogenesis of type 2 diabetes in Taiwanese.

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