- 期刊
Metabolic Changes in the Rat CNS Endogenous Opioid Systems Associated with Electroacupuncture-Produced Analgesia
低頻電針止痛引起老鼠中樞內生嗎啡系統葡萄糖代謝率的改變
摘要
Thirteen adult male Sprague-Dawley rats received one of four different somesthetic stimuli for 45-minutes following an injection of (14C)-2-deoxyglucose (2DG): 1. Non-noxious (40℃ water) distal-third of tail stimulation (Control, n=3); 2. Noxious stimulation (56℃ water) of the distal-third of tail (”Pain”, n=4); 3. Paired noxious tail stimulation and electroacupuncture (EA) (2Hz) of right Tsu-San-Li point (proximal anterior tibial muscle) (”Pain” + EA, n=3); and 4. EA stimulation of right Tsu-San-Li (EA, n=3). Efficacy of noxious thermal and EA stimuli was determined by measuring Tail-flick Respones Latencies. Quantitative 2DG method of Sokoloff et al. (1977) was used to assess functional changes in the central nervous system. The increased Mean Tail-flick Latency (MTFL) in ”Pain” + EA group from 2.61 sec (before EA) to 3.66 sec (after EA) indicated that EA was effective in producing analgesia. In comparison with the Control group, LCGU's in the ”Pair” +EA group were significantly increased (p＜0.05) in the following nuclei: Lateral Reticular nucleus (LRN); Arcuate nucleus (ARC); Paraventricular nucleus of hypothalamus (PVH); Lateral Habenular nucleus (HB); Anteroventral and Anteromedial nuclei of thalamus (AV & AM ); Posterior Cingulate Gyrus (PCG); Medial Mammillary nucleus (MEM) and Anterior Pituitary (ANP). LCGU's in ARC, AV & AM, PCG of the ”Pain” group, however, were significantly increased (p＜0.05 ) in comparison with control groups. In conclusion, EA under the above stimulus conditions activates structures involved with portions of three endogenous opioid analgesia systems 1. Proopiomelanocortin (ANP, ARC, LRN), 2. Proenkephalin (MEM, LRN, PVH), and 3. Prodynorphin (MEM, AV & AM, PCG, HB) derived peptide containing immunoreactive nuclear groups.
關鍵字
無資料並列摘要
Thirteen adult male Sprague-Dawley rats received one of four different somesthetic stimuli for 45-minutes following an injection of (14C)-2-deoxyglucose (2DG): 1. Non-noxious (40℃ water) distal-third of tail stimulation (Control, n=3); 2. Noxious stimulation (56℃ water) of the distal-third of tail (”Pain”, n=4); 3. Paired noxious tail stimulation and electroacupuncture (EA) (2Hz) of right Tsu-San-Li point (proximal anterior tibial muscle) (”Pain” + EA, n=3); and 4. EA stimulation of right Tsu-San-Li (EA, n=3). Efficacy of noxious thermal and EA stimuli was determined by measuring Tail-flick Respones Latencies. Quantitative 2DG method of Sokoloff et al. (1977) was used to assess functional changes in the central nervous system. The increased Mean Tail-flick Latency (MTFL) in ”Pain” + EA group from 2.61 sec (before EA) to 3.66 sec (after EA) indicated that EA was effective in producing analgesia. In comparison with the Control group, LCGU's in the ”Pair” +EA group were significantly increased (p＜0.05) in the following nuclei: Lateral Reticular nucleus (LRN); Arcuate nucleus (ARC); Paraventricular nucleus of hypothalamus (PVH); Lateral Habenular nucleus (HB); Anteroventral and Anteromedial nuclei of thalamus (AV & AM ); Posterior Cingulate Gyrus (PCG); Medial Mammillary nucleus (MEM) and Anterior Pituitary (ANP). LCGU's in ARC, AV & AM, PCG of the ”Pain” group, however, were significantly increased (p＜0.05 ) in comparison with control groups. In conclusion, EA under the above stimulus conditions activates structures involved with portions of three endogenous opioid analgesia systems 1. Proopiomelanocortin (ANP, ARC, LRN), 2. Proenkephalin (MEM, LRN, PVH), and 3. Prodynorphin (MEM, AV & AM, PCG, HB) derived peptide containing immunoreactive nuclear groups.