- 期刊
地黃成分分析方法之開發及以醣類指標作生熟地黃之辨別
A Method of Analyzing the Composition of Rehmannia and Using Carbohydrate Indicators for Differentiating Rehmanniae Radix and Rehmanniae Preparata Radix
摘要
地黃為常用中藥材，依其加工處理程序的不同，而區分為生地黃與熟地黃，但兩者的藥性與臨床應用完全不同。本文以Hypersil Hypercarb (porous graphitic carbon)作為分離管柱，串聯UV及ELSD檢測器，使用0.1%甲酸水溶液及甲醇為流動相，於60分鐘內可同時分析生地黃中的catalpol (9)、熟地黃中的5-Hydroxymethylfurfural (5-HMF) (8)，及地黃所屬的各種醣類成分glucose (1)、sucrose (2)、melibiose (3)、manninotriose (4)、raffinose (5)、stachyose(6)、verbascose (7)共計9種化合物的含量。本分析方法有良好的再現性，以ELSD偵測之化合物1~7，其波峰積分面積（或面積比）之RSD值介於1.01~3.38%（同日）及1.24~3.33%（異日）之間。以UV偵測之化合物8、9，其積分面積RSD值同日為1.01、1.23%，異日為1.24、1.52%。生地黃中主要醣類為stachyose (6)，熟地黃則為manninotroiose (4)，我們用14批生地黃與13批熟地黃樣品的分析數據導出判定地黃炮製程度的醣類指標A(9(上标 *)[4/(4+6)])與指標B(log(4/6))，經統計後發現，當地黃樣品的指標A數值落在0~1.57之間，或指標B數值落在－2.42~-0.34之間時，該樣品為生地黃；如受測地黃樣品的指標A數值落在8.09~9之間，或醣類指標B數值落在1.04~3.28之間時，該樣品為炮製良好之熟地黃。
並列摘要
Rehmannia is a commonly used Chinese medicine. According to different processing procedures, Rehamnnia is classified as Rehmanniae Radix and Rehmanniae Preparata Radix that have totally different pharmaceutical properties and clinical applications. The present study used Hypersil Hypercarb (porous graphitic carbon) as the column connected to the UV and ELSD detectors. Using 0.1% formic acid aqueous solution and methanol as the mobile phase, the composition of a total of 9 compounds, catalpol (9) in Rehmanniae Radix, 5-hydroxymethylfurfural (5-HMF) (8) in Rehmanniae Preparata Radix, and the various carbohydrates including glucose (1), sucrose (2), melibiose (3), manninotriose (4), raffinose (5), stachyose (6), and verbascose (7) in Radix were analyzed simultaneously within 60 minutes. This analytical method showed good reproducibility. The RSD values of the peak areas of the analysis of compounds 1~7 by ELSD ranged between 1.01~3.38% (intraday) and 1.24~3.33% (interday). The RSD values of the analysis of 8 and 9 were 1.01% and 1.23% (intraday) and 1.24% and 1.52% (interday). The major carbohydrates in Rehmanniae Radix and Rehmanniae Preparata Radix were determined to be stachyose (6) and manninotroiose (4), respectively. The data of the samples from 14 batches of Rehmanniae Radix and 13 batches of Rehmanniae Preparata Radix were used to determine the processing of Rehamnnia by indicators A (9*[4/(4+6)]) and B (log(4/6)). Based on the statistical data, the indicators A of 0~1.57 and B of -2.42~-0.34 indicated that the sample was collected from Rehmanniae Radix. On the other hand, the indicators A of 8.09~9 and B of 1.04~3.28 indicated that the sample was collected from the well processed Rehmanniae Preparata Radix.