國家同步輻射研究中心高效能蛋白質結晶學設施之發展現況

Current Status of Crystallography Beamlines and End Stations at NSRRC

趙俊雄(Chun-Hsiung Chao) ； 簡玉成(Yuch-Cheng Jean)

《科儀新知》 150期 (2006/02) Pp. 44-49

https://doi.org/10.29662/IT.200602.0005

摘要

蛋白質結晶學是鑑定生物分子三度空間結構的最有力工具之一，配合同步輻射光的高亮度及能量可調性，可以大幅提昇生物分子的結構鑑定速度，足以滿足基因體學及藥物設計等需要大量分子結構資料等研究的需求。為此，國家同步輻射研究中心特別興建兩座高效能蛋白質結晶學光束線，其中一座為針對未知蛋白質結構解析用途，能量範圍從6.5至19仟電子伏特(keV)之多波長異常繞射(MAD)光束線，另一座為供晶體篩選、藥物設計以及高解析度結構分析用，能量為12至14仟電子伏特之單色光(mono)光束線。使用最成熟可靠的光束線技術，配備最先進的面積偵測器系統、高速高效能的計算及網路環境、自動化的樣本裝卸系統，及友善的數據收集與控制系統，MAD光束線一年約可收集800組MAD數據，單色光光束線一年約可收集1200組高解析度數據。

並列摘要

Protein crystallography is one of the most powerful and dominant tools to determine the three-dimensional structures of biological macromolecules today. In order to increase the rate of crystal structure determination, the highly brilliant and easily tunable synchrotron radiation (SR) X-ray source is essential to meet the needs of abound structural information for studying structural genomics and structural-based drug designs. To satisfy these requirements, the National Synchrotron Radiation Research Center (NSRRC) has constructed two dedicated Synchrotron Radiation Protein Crystallography Facility (SPXF) high-throughput beamlines for structural genomics research. One of the beamlines will be a multi-wavelength anomalous diffraction (MAD) beamline with energy tunability from 6.5keV to 19keV for unknown structure determinations, and the other will be a monochromatic (mono) beamline with energy from 12keV to 14keV for crystal screening, drug design and high-resolution structure studies. The well-developed light source technique, advanced area detectors, high-efficient computing and network environments, automatic crystal sample changing and centering, and user-friendly data collection and control systems will produce 800 MAD data set per year for MAD beamline and 1200 useful data sets per year for mono beamline.