用於藥物測試之灌流式三維細胞培養微流體晶片

Perfusion 3-D Cell Culture Microfluidic Chip for Drug Testing

吳旻憲(Min-Hsien Wu)

《科儀新知》 239期 (2024/06) Pp. 64-74

摘要

能忠實地反映出藥物在人體反應的體外細胞測試模式，不僅能避免實驗動物的使用，這種仿生模式亦能節省藥物研究的時間和成本。為此，利用微流體晶片來進行灌流式三維細胞培養被認為具有潛力。本文章首先將提供一些背景資訊，其包含細胞培養在藥物研發的角色、灌流式三維細胞培養的意義、利用微流體技術來執行這種細胞培養的優缺點及建構這種細胞培養模式需考量的議題。最後，本文章將介紹一項簡單且易操作的灌流式三維細胞培養系統。

並列摘要

An in vitro cell culture model that can faithfully reflect the response of drugs in the human body not only avoids the use of experimental animals, but this biomimetic model can also save time and cost in drug research. To achieve this goal, the use of microfluidic chips for perfusion-based three-dimensional (3-D) cell culture is considered to have potential. This article will first provide some background information, including the role of cell culture in drug development, the significance of perfusion 3D cell culture, the advantages and disadvantages of using microfluidic technology to perform this type of cell culture, and the considerations for constructing this cell culture model. Finally, this article will introduce a simple and easy-to-operate microfluidic-based perfusion 3-D cell culture system.