- 期刊
綠茶兒茶素給予F344大鼠攝食90天的毒性評估
Evaluation of Toxicity of Green Tea Catechins with 90-day Dietary Administration to F344 Rats
摘要
綠茶兒茶素(green tea catechin, GTC)，為由Camellia sinensis之梗與葉中萃取出來之多酚類(polyphenols)，在不同型式之茶飲料中均可以發現可對多種食物、點心、脂肪和脂肪油，被當作抗氧化性添加劑(antioxidant additives)。基於其安全性評估之一部分，我們將濃度分別為0（控制組）、0.3%、1.25%與5%之食用性GTC給male雄性與雌性F344大鼠共90天，來探討其亞慢性毒性。此種濃度下，每組之大鼠平均每天GTC之攝取量為180、764與3525 mg/kg BW（0.3%、1.25%與5%），給雄性大鼠，而189、820與3542 mg/kg BW給雌性大鼠。在整個實驗期間，並無死亡或造成明顯之臨床病徵發生，但在5%之實驗雄性大鼠中，由第一週起到實驗終了時，有體重的下降。在血清生化檢查中，alanine transaminase與alkaline phosphatase在給5%GTC之雄性與雌性大鼠中及aspartate transaminase之濃度在給5% GTC之雌性大鼠中會升高。與兩種性別接受5% GTC處理者之相關肝臟重量增加有關。雖然在接受0.3~5.0% GTC之雄性大鼠中，總膽固醇會降低，且在接受1.25%與5.0% GTC之雄性大鼠與5.0% GTC之雌性大鼠之中性脂肪均會減低，可是這些改變並不被認為是不良副作用。其血液學與由組織病理學之結果發現，並無GTC相關之毒性改變出現。基於上述之發現，被評估給1.25%的GTC（即給雄性大鼠764 mg/kg BW及820 mg/kg BW每日給雌性大鼠）之結果並無觀察到副作用的程度(no obseved adverse effect level, NOAEL)。
關鍵字
無資料並列摘要
Green tea catechins (GTC), polyphenols extracted from the stalks and leaves of Camellia sinensis, are found in the different types of tea beverages and as antioxidant additives to many foods, snacks, fats and fatty oils. As a part of their safety assessment, subchronic toxicity was investigated in male and female F344 rats with dietary administration at concentrations of 0 (control), 0.3%, 1.25% and 5.0% for 90 days. The average daily intakes of GTC in each group were 180, 764 and 3525 mg/kg body weight/day, respectively for males, and 189, 820 and 3542 mg/kg body weight/day, respectively for females. No mortality or obvious clinical signs were observed throughout the experimental period but body weights were reduced from week 1 to the end of the experiment in 5.0% males. In serum biochemistry, alanine transaminase and alkaline phosphatase in 5.0% males and females and aspartate transaminase in 5.0% females were increased, together with the relative liver weights in both sexes receiving 5.0%. Although decreases were evident for total cholesterol in 0.3-5.0% males and triglycerides in 1.25% and 5.0% males and 5.0% females, these changes were not considered to be adverse. Hematology and histopathological observation revealed no GTC-related toxicological changes. Based on above findings, the no observed adverse effect level (NOAEL) of GTC was estimated to be 1.25% (764 mg/kg body weight/day for males and 820 mg/kg body weight/day for females).