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隱形鏡片污染產生Glycocalyx之定量－評估細菌黏著之實驗模式
Quantitation of Glycocalyx in Contact Lens Contamination-An Experimental Model in Evaluation of Bacterial Adherence
摘要
Glycocalyx是細菌產生之多醣類聚合體，細菌藉此保護性生物薄膜互相黏連，同時附著於生物材料如隱形眼鏡等。隱形眼鏡之細菌黏著污染，可造成角膜潰瘍；而有關細菌黏著之研究，以往需藉助於掃瞄式電子顯微鏡，放射性同位素活性測定等昂貴之設備。本研究以分光度計檢法定量實驗上綠膿桿菌污染鏡片產生之glycocalyx，同時使用掃瞄式電子顯微鏡觀察，並計算黏著細菌數，以評估並比較兩種檢查方法之價值。結果顯示定量glycocalyx可部分反應細菌黏著於污染鏡片之程度，但仍有必要進一步之研究；而掃瞄式電子顯微鏡雖可實際計算細菌之平均黏著數，然標準誤差極大，且相當耗時，其實用性需再加以評估。
關鍵字
無資料並列摘要
Bacterial adherence to the contact lens is a major step in the pathogenesis of contact lensrelated keratitis. Glycocalyx, a polyanionic acid mucopolysaccharide secreted by the bacteria, plays an important role in the bacterial adherence to biomaterials such as contact lenses. In the previous research of bacterial adherence to contact lenses. Previous research on bacterial adherence to contact lenses required expensive equipments as scanning electron microscopy (SEM), and radioactivity assay. However, recent development in quantitation of bacterial glycocalyx with spectrophotometry appears to be an alternative in evaluating bacterial adherence to contact lenses. In the current study, hydrophilic contact lenses were co-cultured with various concentrations of Pseudomonas aeruginosa solution at different time intervals. Glycocalyces were quantitated with spectrophotometry after toluidinc blue staining, and density of adhered bacteria was quantitated with SEM. It was concluded that the degree of bacterial adherence on the contaminated contact lenses could be correlated, to some extent, to the amount of glycocalyx. Traditional quantitation of the density of adhered bacteria with SEM was impractical because the distributions of bacteria were not uniform and calculations of adherence density were time-consuming. The authors suggested that although quantitation of glycocalyx with spectrophotometry can be valuable in understanding the process of contact lens contamination, further studies are necessary to elucidate the value of its clinical use.