From year 2004 to 2013, a total of 690 suspected cholera cases were reported in Taiwan. Among them, 323 cases were reported through "cholera" disease in notifiable disease surveillance system, the remaining 367 cases without stool or vomit specimens, were reported as "others" disease in the same system. Among 515 cases positive for Vibrio cholerae, only 28 cases were toxigenic cholera. The trends of Vibrio cholerae positive cases increased in recent years, particularly increased from May to October, more than half of cases occurred in summer season. The sex ratio of "Vibrio cholerae" positive cases is 1.7 times, majority of the cases were ≥50 years-old and raised yearly, and most of cases lived in the southern region. A large number of "Vibrio cholerae" positive cases (456) were either Non-01 or Non-0139, of which 169 cases were reported through "cholera" disease; O1-Ogawa were 54 cases, of which only 2 cases were reported through "others" disease. Over the 10 years period, there were merely two toxigenic 0139 cases detected in year of 2006 and 2010. Currently, due to socio-economic development and public health improvement, cholera only happened sporadically in Taiwan. Taiwan is a subtropical island and surrounded by sea, aqua farming becomes more popular, plus the habit and easy access of seafood from the public, people might be infected with "Vibrio cholerae" and caused sepsis or even death. Therefore, public health authorities not only should strengthen on cholera surveillance, investigation and prevention measures, but also to explore the resistance and toxigenicity of Non-01/Non-0139 "Vibrio cholerae" serotypes.