- 期刊
Compound 48/80-induced Degranulation of GnRH-like Immunoreactive Mast Cells in the Brain and Mesentery of the Gerbil
化合物48/80誘發的沙鼠腦及腸繫膜內具GnRH免疫反應之肥大細胞的去顆粒化
摘要
肥大細胞可經由去顆粒化（degranulation）而影響其細胞外環境。近來的研究結果顯示，鴿子及哺乳類的腦肥大細胞可表現促性腺激素釋放激素（Gonadotropin-releasing hormone, GnRH）的免疫反應。本研究的目的在探討GnRH於腦及腸繫膜肥大細胞的表現，以及投予化合物48/80後其去顆粒化情形。本研究採用沙鼠為實驗動物，因沙鼠的腦及腸繫膜內具有大量肥大細胞。本研究之結果顯示：腦及腸系膜的肥大細胞，不論是對照組或投藥組皆可表現GnRH的免疫反應。腸繫膜肥大細胞於腹腔內投藥後有非常劇烈的去顆粒化反應。電子顯微鏡觀察結果顯示，沙鼠之腦及腸繫膜肥大細胞所釋放具有GnRH免疫反應的顆粒都相當緻密，且釋放顆粒後的肥大細胞可於投藥後十二小時復原。由上述結果可得以下結論：（一）腦及腸繫膜肥大細胞皆可表現GnRH的免疫反應，以及（二）GnRH是以快速的釋放反應經由肥大細胞釋放出來。
並列摘要
Mast cells affect the extracellular milieu through the process of degranulation. Recently, mast cells in the brain of the dove and certain mammals were found to express gonadotropin-releasing hormone (GnRH)-like immunoreactivity (IR). In the present study, we examine the expression of GnRH-like IR in brain and mesenteric mast cells, and the degranulation of mast cells at these 2 sites after administration of compound 48/80 (C48/80). The gerbil, in which numerous mast cells are found in the brain and mesentery, was used as the experimental animal model. Both brain and mesenteric mast cells in control and C48/80-treated gerbils express GnRH-like IR. Mast cell degranulation was dramatic in the mesentery after C48/80 was injected intraperitoneally. Because newly extruded GnRH-like immunoreactive granules in the brain and mesentery are compact, GnRH release can be classed as a rapid-release reaction. The degranulated mast cells were restored to their normal configuration in the brain and mesentery 12 h after C48/80 treatment. We conclude that: (1) brain and mesenteric mast cells express GnRH-like IR, and (2) GnRH is released by a rapid-release reaction after C48/80 administration.