- 期刊
油脂中五種合成抗氣化劑之系統分析
Simultaneous Determination of Five Synthetic Antioxidants in Fats and Oils
摘要
利用逆相高效液相層析法(RP-HPLC)，分析食用油脂中沒食子酸丙酯(PG)、第三丁基氫醌(TBHQ)、NDGA、丁基羥基甲氧苯(BHA)及二丁基羥基甲苯(BHT)五種抗氧化劑之系統檢驗方法業已經本研究建立。其主要分析步驟為檢品溶於正戊烷，取一定量注入固相抽出分離管(Adsorbex RP-18)並收集流出液。該固相抽出分離管再用溶離液(2-Propanol/Acetonitrile=1:1)每次1ml分別沖洗三次收集溶出液；合併流出液及溶出液並以溶離液加至4ml供作檢液。注入定量之檢液於逆相高效液相層析儀中（管柱μ-Bondapak C18）同時做五種合成抗氧化劑之定量分析。 取一定量之沒食子酸丙酯、第三丁基氫醌、NDGA、丁基羥基甲氧苯及二丁基羥基甲苯標準品混合溶解後，分別添加於各種食用油脂中，經測試結果其回收率分別為92.0~98.1%，92.2~99.9%，98.8~100.7%，90.5~97.1%及91.0~97.0%。 上述五種抗氧化劑其濃度範圍在2.5~20μg/ml範圍內，任取五種濃度加以測試，其標準曲線及檢量線均能維持良好之線性關性。本系統檢驗法不僅操作簡便，溶媒的使用量大減，並具良好的線性關係及回收率，在對食用油脂進行上述五種抗氧化之同時分析時效果亦十分卓越。
關鍵字
無資料並列摘要
A high-performance liquid chromatographic (HPLC) method for simultaneous determination of gallic acid n-propyl ester (PG), tert-butyl hydroquinone (TBHQ), nor-dihydroquaiaretic acid (NDGA), 3-tert-butyl-4-hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in fats and oils was developed. The samples were dissolved in n-pentane. Aliquot (1 ml) of the pentane solutions was pipetted onto Adsordex RP-18 extraction column (400 mg). The antioxidants were eluted with three 1-mi portions of acetonitrile/2-propanol (1:1). The filtrate and the eluate were collected and mixed. The final volume was adjusted to 4 ml with acetonitrile/2-propanol and determined by HPLC with a UV detector set at 280 nm. The recoveries of PG, TBHQ, NDGA, BHA and BHT fortified to various edible fats and oils were in the range of 92.0~98.1%, 92.2~99.9%, 98.8~100.7%, 90.5~97.1% and 91.0~97.0%, respectively. Each of the standard and calibration curves for PG, TBHQ, NDGA, BHA and BHT showed a good linear relationship in the range of 2.5~20 ppm.