- 期刊
Determination of Codeine, Morphine and 6-Acetylmorphine in Urine
尿中可待因、嗎啡與單乙醯嗎啡之簡易分析法
摘要
本研究開發並評估－簡易的氣相層析／質譜儀方法，供定量尿中free codeine, free morphine, 6-acetylmorphine, total codeine, total morphine。抽取用之緩衝液以pH值為9.0時，其抽取回收率最高。抽取回收率，free codeine為98.3%，free morphine為76.7%，6-acetylmorPhine為85.6%，三甲基矽化衍生物在七十二小時內均非常安定，適合其定量分析。nalorphine為內標準品，以SIM (Selected Ion Monitoring)方式定量。濃度在50至1000ng/mL範圍內均具有良好的線性關係，日內及日間精密度試驗顯示，codeine的變異係數為0.47~7.72%，morphine為0.31~8.09%，6-acetylmorphine為0.49~6.15%。
並列摘要
A simple yet effective GCIMS protocol was developed and evaluated for the determination of free codeine, free morphine, 6-acetylmorphine, total codeine and total morphine in urine. Optimal recovery was achieved with extraction conducted at pH 9.0. Trimethylsilyl-derivatives of these analytes and the internal standard (nalorphine) were found adequately stable for a 72-hour period. Quantitative determination of the analytes was performed by selective ion monitoring. Excellent linearity was observed over the 50-1000ng/mL concentration range studied. The intraday and interday precisions range from 0.47 to 7.72% for codeine, 0.31 to 8.09% for morphine and 0.49 to 6.15% for 6-acetylmorphine. The overall recoveries for codeine, morphine and 6-acetylmorphine were found to be 98.3%, 76.7% and 85.6% respectively.