- 期刊
Infection of Cotton Pollen by Verticillium Dahliae
棉花黃萎病菌(Verticillium dahliae)侵染花粉
摘要
利用光學顯微鏡及電子顯微鏡觀察來研究棉花花粉與棉花黃萎病(Verticillium dahliae)的關係，結果顯示新鮮花粉的水溶性浸出液能促進黃萎病菌分生孢子的發芽管的生長。將新鮮花粉與分生孢子混合培養４天，以顯微鏡觀察，顯示棉花花粉易受黃萎病菌侵染。入侵過程是由菌絲頂端直接穿透花粉細胞的外壁(Exine)與內壁(Intine)，進而使菌絲在細胞內大量繁殖，終至造成花粉細胞質崩潰解體。在每一菌絲入侵部位均會造成細胞內壁澎大，以及內、外壁受病原菌切割及融解的現象。此種帶菌花粉在棉花黃萎病流行病學上的重要性也在本文加以討論。
並列摘要
A light- and electron-microscopic study revealed that the presence of cotton pollen grains or cotton pollen diffusates enhanced the growth of germ tubes of Verticillium dahliae, the causal agent of Verticillium ilt of cotton. Microscopic examinations of 4-day-old samples of pollen- spore mixtures revealed that cotton Jollen grains were susceptible to infection by V. dahliae. Infection of pollen grains occurred by direct hyphal tip penetration of the pollen cell walls, ramification of invaded hyphae in the cell lumen, and disintegration of pollen cytoplasm. Sharp etching and cleavage of both exine and intine layers of pollen cell walls were evident at the penetration sites. The potential impact of V. dahliae-infected pollen on the epidemiology of Verticillium wilt of cotton was also discussed.