- 期刊
一種與大理花嵌紋病相關之花椰菜嵌紋病毒之分之特性分析
Molecular Characterization of a Dahlia Mosaic-Associated Caulimovirus
摘要
大理花（Dahlia pinnata or D. variabilis）葉片呈現嵌紋、黃脈與葉脈鑲帶等疑似病毒感染之病徵。電子顯微鏡觀察可發現罹病組織粗汁液含有直徑約45nm的球形病毒顆粒，也可在罹病葉部細胞內觀察到花椰菜嵌紋病毒屬（Caulimovirus）典型的內含體，研判疑由大理花嵌紋病相關病毒所引起。萃取罹病大理花組織總量DNA，配合大理花嵌紋病相關病毒各基因的引子對進行PCR反應。選殖各段基因的PCR產物並進行核苷酸序列解析。結果顯示病毒基因組的長度（7942 bp）和結構（6 ORF）與Caulimovirus類似，而核苷酸序列則與Dahlia common mosaic virus-New Zealand （DCMV-NZ）完整序列有高達98.3%的相同度。各個ORFs的核苷酸序列與胺基酸序列亦都和DCMV-NZ以及Dahlia mosaic virus-Holland （DMV-Holland）有90%以上的相同度；但和同是大理花嵌紋病相關的DMV-Portland及DMV-D10則有明顯的差異性。本研究解析台灣地區一個大理花嵌紋病相關之病毒的分子特性，證實該病毒為大理花普通嵌紋病毒的台灣分離株（Dahlia common mosaic virus Taiwan isolate，DCMV-TW）。
並列摘要
Dahlia plants (Dahlia pinnata or D. variabilis) with symptoms of mosaic, chlorotic veins, and vein banding on leaves were collected in Taipei area in 2011. Both virus-like particles with diameter of about 45-50 nm and typical inclusion bodies of Caulimovirus wereobserved in the sections of diseased leaf tissues by electron microscopy, indicating the infection of a caulimovirus in dahlia plants. We amplified, cloned, and determined the sequences of the virus. The full-length genome (7942 bp) of the virus consisted of 6 major open reading frames (6 ORFs). Comparisons based on nucleotide and amino acid sequences of each ORF with that of members of Caulimovirus showed that the studied virus is a Taiwan isolate of the Dahlia common mosaic virus (DCMV-TW).