- 期刊
木質纖維素廢棄物酵素醣化技術之開發
Enzymatic Saccharification of Lignocellulosic Materials to Sugars
摘要
木質纖維素（Lignocellulose）是地球上最豐富的有機物，在本論文中探討生產木質纖維分解酵素的方法，並利用酵素處理法水解由草坪收集之木質纖維素廢棄物，將雜草木質纖維素成功轉變為葡萄糖、木糖與阿拉伯糖…等。實驗中以里氏木黴菌（Trichoderma reesei）為對象，經液態發酵法培養木質纖維分解酵素生產，探討最適生產木質纖維分解酵素之條件，搖瓶實驗發現，加入纖維素及麩皮的實驗組最佳，濾紙分解活性達0.84 FPU/mL、外切型酵素活性達0.23 U/mL、內切型酵素活性達7.52 U/mL、纖維二醣分解酵素活性達3.26U/mL、半纖維分解酵素活性達61.86 U/mL。再依最適條件擴大培養於6.5 L小型醱酵槽生產該酵素群，分析濾紙分解活性最終達0.46 FPU/mL。將所生產木質纖維分解酵素，應用於木質纖維素醣化技術之開發，比較商業酵素及自行生產之酵素以測試雜草木質纖維素醣化效率，最佳組合可將1公克雜草廢棄物轉化成醣類比例達248毫克，轉化率達24.8%。此研究提供了一個新方法，可以將雜草木質纖維素水解成醣水，展望未來，將醣水經由微生物發酵生產單細胞蛋白、酒精、丁醇或其他高附加價值產品。
並列摘要
Lignocellulosic materials are the most abundant organic mass in the biosphere. The methods of lignocellulase production and the conversion rates of the enzymatic saccharification technology for garden weeds converted into D-glucose, D-xylose and Darabinose were investigated. By analyzing lignocellulases produced in different substrate inductions, the results showed that both cellulose and bran with appropriate amounts could accelerate the production rate by Trichoderma reesei. In the best shake flask scale, it was found that the filter paper actvity was 0.84 FPU/mL, avicelase activity 0.23 U/mL, endoglucanase activity 7.52 U/mL, salicinase activity 3.26 U/mL and xylanase activity 61.86 U/mL. In a up-scaling 6.5 L ferwenter, the results showed that the filter paper activity is 0.46 FPU/mL. With optimized conditions, I gram weeds could be converted into 248 mg sugars. The conversion rate reached to 24.8%. Those converted sugars could be further transformed into single cell protein, ethanol, butanol or other useful products by different microorganisms.