The healthy worker effect (HWE) is an important issue in occupational epidemiological studies. It refers to an observed decrease in mortality or morbidity in workers when compared with the general population. Sources of HWE can be classified into four categories: true effect and selection, information, and confounding biases. The magnitude of HWE is estimated to bias the mortality of the workers by 10% to 30%. Employment duration, specific cause of death, type of occupation, socio-economic status, age and sex were identified as contributory factors of HWE. These factors could be controlled in the process of research design or data analysis to minimize the HWE. No matter what type of studies and what kind of data to be collected, HWE should be kept in mind while conducting occupational epidemiological studies, and strategies should be taken to reduce its interference with the interpretation of research finding.