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以RAPDs分子標誌鑑定臺灣原生蝴蝶蘭親緣關係之研究
Random Amplified Polymorphic DNA (RAPD) for Genetic Analysis of Taiwan Native Phalaenopsis Cultivars
摘要
本研究以逢機增幅多型性DNA（RAPD）方法，探討原生種蝴蝶蘭演化的親緣關係，結果顯示用八種臺灣原生種的蝴蝶蘭與菲律賓的阿媽品種為材料，利用24種引子所產生之DNA條帶分析時，依據RAPD相似性係數所得到之樹狀圖及園藝性狀所得親緣關係，結果顯示以CT81/CT82/CT85/CT86為引子，測試的原生種蝴蝶蘭可分為三群，第一群為T1、T2、T3、T5，第二群為T4、T6、T7、T8，另一群為T9，如以CT81/CT85/CT86為引子，所得結果亦分三群，第一群為T1、T4、T6、T7、T8，第二群為T2、T3、T5，第三群為T9，且T9與其他8個臺灣原生品種相似度僅0.16，可以明顯分出來自菲律賓的阿媽T9與其他8個採自本地的臺灣原生蝴蝶蘭屬於不同品種。
並列摘要
A Randam Amplified Polymorphic DNA (RAPD) analysis was conducted to study the Phylogenetic relationship of taxonomy and evolution among 8 Taiwna native cultivars of Phalaenopsis and P. amabilis. Results showed stable similarity coefficient and relative order after 24 primers had been analyzed. In addition, the result derived from the comparison either dendrogram generated by RAPD or morphological characteristic traits idemtifiedthat the evolutionary tendency of Phalaenopsis. Its origin seemsed to be polyphyletic. The dendrogram resulting from unweight pair group method with arithmetic averages cluster analysis using the NTSYS program separated the native Phalaenopsis into three groups by using CT81/CT82/CT85/CT86 primer. The first group had four species of T1, T2, T3, T5. The second group includes T4, T6, T7, T8, the third group T9. Otherwise using CT81/CT85/CT86 primers, also separated native Phalaenopsis into three groups. The first group had five cultivars of T1, T4, T6, T7, T8, and the second group includes T2, T3, T5, while, the T9 (P. amabilis from Phillipine) named the thrid group Basically the collecting 8 Taiwan native Phalaenopsis all showed great differentiation from P. amabilis of Phillipine.