蒙地卡羅法分析硼在細胞內的頒佈

Subcellular Distribution of Various Boron Compounds by Monte Carlo Simulation

趙良曉(Liung-Sheau Chao) ； 陳光耀(Kuang Y. Chen)

《放射治療與腫瘤學》 1卷2期 (1994/06) Pp. 173-178

https://doi.org/10.6316/TRO/199401(2)173

摘要

自從尋癌藥物能選擇性的進入細胞後，硼捉中子治療法在癌症治療的前景被看好。此種方法的關鍵療效在於靶細胞中硼原子的濃度分布狀況。評估及量測的方法很多，例如：離子顯微術法，核磁共振影像法等。在此探討蒙地卡羅法分析硼在細胞內的分佈。在進行模擬之前必須先界定何種藥物進入何種細胞，并利用無燄原子吸收光譜儀將實驗數據獲得。例如：百萬細胞中硼的含量，各時期細胞器（粒腺體、溶解體、細胞核、內質網及細胞質）中硼含量的百分比，利用測得的數據進行模擬後可以判別何種藥物的潛在效力最佳。本報告分別討論細胞珠、硼化合物、無燄原子吸收光譜儀、細胞吸收、次細胞分布及蒙地卡羅法。次細胞分布研究中已證明大部分的硼原子頒佈在細胞質中，而最多的硼濃度是存在於溶解體中，而核中的硼濃度較低。紫質類的復合物BOPP在細胞中有較多的硼原子含量，經由蒙地卡羅法的證明BOPP擁有最高的潛在效力。

並列摘要

The boron neutron capture therapy has a promising role in cancer treatment since it can achieve selectivity at the cellular level. The effect of this therapy depends on the subcellular localization of boron atoms in the target cell. There were many method to evaluate it. For example ion microscropy or nuclear magnetic resonance image method. Now, We study the subcellular distribution of various boron compounds by Monte Carlo simulations. At first, We should confirm that some kinds of tumor seeking agent into the special cell lines and measured the experimental data by flameless atom absorption spectrophotometry. For example. The boron uptake in one million cells, The percentage of boron content in organelle (mitochondria, lysosomes, endoplasimic reticulum and cytoplasm) during every stages. We could determined which drug has the best potential effectiveness. In this study we divided the following section: Cell lines, Boron Compounds, Cellular Uptake. Subcellular fraction studies showed that most of the boron localized in the cytoplasm of the cells. A significantly higher boron concentration was found in the lysosomes. BOFF is porphyrin complexes and it can produces a much higher intracellular level of boron than the other compounds. Monte Carlo simulation code has showed that BOPP would yield the highest potential effectiveness.